10.1002/jlcr.2580340213
The research aims to develop a method for synthesizing 8-[18O]hydroxy-2'-deoxyguanosine (8-[18O]OH-dG) to be used as an internal standard for the quantification of 8-hydroxy-2'-deoxyguanosine (oh8dG) by GC-MS. oh8dG is a significant marker of oxidatively damaged DNA, which is relevant in carcinogenesis, mutagenesis, and toxicology. The synthesis process involves several steps, starting with the bromination of 2'-deoxyguanosine to produce 8-bromo-2'-deoxyguanosine, followed by the conversion to 8-[18O]benzyloxy-2'-deoxyguanosine using the sodium salt of [18O]benzyl alcohol, and finally, the reduction to 8-[18O]hydroxy-2'-deoxyguanosine through catalytic transfer hydrogenation. Key chemicals used include 2'-deoxyguanosine, bromine, ethyl benzimidate hydrochloride, H218O, LiAlH4, sodium hydride, and palladium on carbon. The overall yield of 8-[18O]OH-dG from H218O was approximately 4%, with an isotopic purity of 93.4 atom% as determined by GC-MS. The synthesized compound meets the required purity standards for its intended application as an internal standard in the analysis of oh8dG extracted from in vivo DNA and urine.