10.1002/jhet.2341
This research investigates the synthesis of hexahydroquinolines and their fused derivatives through the reaction of enamines with substituted cinnamonitriles, using DABCO, piperidine, or chitosan as catalysts. The purpose is to develop an efficient and environmentally benign method for synthesizing these biologically significant compounds, which have applications in the pharmaceutical field, particularly for their antibacterial and anticancer activities. The study concludes that DABCO and chitosan are highly effective catalysts, with chitosan being a green alternative. The structures of the synthesized compounds were unambiguously determined using 2D-HMBC NMR spectroscopy, confirming the regioorientation of the Michael addition reaction.
10.1016/j.bmc.2009.11.013
The research describes the development of a novel anti-HIV polymeric prodrug: chitosan-O-isopropyl-50-O-d4T monophosphate conjugate. The study aims to improve the antiviral efficacy of nucleoside reverse transcriptase inhibitors (NRTIs) and reduce their side effects by constructing a nanosized NRTI monophosphate-polymer conjugate using d4T as a model NRTI. Key chemicals involved in the research include chitosan, a biodegradable and biocompatible polysaccharide used as the polymeric vehicle, and d4T (stavudine), an NRTI used in the treatment of HIV infection. The synthesis of the chitosan-d4T conjugate was achieved through the Atherton–Todd reaction under mild conditions, resulting in a water-soluble prodrug with a degree of substitution (DS) of 17.0%. Other chemicals used in the synthesis process include O-isopropyl-5-H-phosphonate of d4T, synthesized using phosphorus trichloride as a phosphorylation reagent, and various reagents such as triethylamine, tetrachloromethane, and sodium tripolyphosphate (TPP) for the preparation of nanoparticles. The study evaluated the anti-HIV activity and cytotoxicity of the conjugate in MT4 cells, prepared nanoparticles for enhanced delivery to viral reservoirs, and conducted in vitro drug release studies to assess the controlled release of d4T monophosphate derivatives.