10.1021/ol016093m
2'-Deoxy-5'-O-(4,4'-dimethoxytrityl)uridine (1) is a protected nucleoside, specifically a protected deoxyuridine. The 5'-hydroxyl group is protected with a 4,4'-dimethoxytrityl (DMT) group, which is commonly used in solid phase oligonucleotide synthesis to prevent unwanted reactions at the 5' position. It serves as a starting material for the synthesis of nucleoside phosphoramidite building blocks. The DMT group can be removed later in the synthesis to allow for further functionalization. 2-Cyanoethyl N,N,N',N'-tetraisopropylphosphoramidite is a phosphoramidite reagent used in the phosphorylation step of nucleoside phosphoramidite synthesis. It is used to convert nucleoside derivatives (2a-d) into the desired phosphoramidites (3a-d) which are then used in machine-assisted DNA synthesis. Succinic anhydride, a cyclic anhydride used for alcohol esterification, was used to convert the nucleoside derivative 2a to the corresponding succinate ester (17), which was then immobilized on a long-chain alkylamine CPG polymer support.