L-Histidine 7-amido-4-methylcoumarin

Base Information

• Chemical Name: L-Histidine 7-amido-4-methylcoumarin
• CAS No.: 191723-64-5
• Molecular Formula: C16H16N4O3
• Molecular Weight: 312.32
• DSSTox Substance ID: DTXSID20426783
• Wikidata: Q82239594
• Mol file: 191723-64-5.mol

Synonyms:L-Histidine 7-amido-4-methylcoumarin;191723-64-5;H-His-AMC trifluoroacetate salt;(S)-2-Amino-3-(1H-imidazol-5-yl)-N-(4-methyl-2-oxo-2H-chromen-7-yl)propanamide;(2S)-2-amino-3-(1H-imidazol-5-yl)-N-(4-methyl-2-oxochromen-7-yl)propanamide;H-His-AMC;L-His-AMC;DTXSID20426783;MFCD00152099;AKOS015911547;AKOS027251073;H-2350;H-HIS-AMC;1H-Imidazole-5-propanamide,a-amino-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-,(as)-

Chemical Property of L-Histidine 7-amido-4-methylcoumarin

Chemical Property:

• Boiling Point: 696 °C at 760 mmHg
• Flash Point: 374.8 °C
• PSA: 114.01000
• Density: 1.413 g/cm³
• LogP: 2.10630
• Storage Temp.: -15°C
• XLogP3: 0.3
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 4
• Exact Mass: 312.12224039
• Heavy Atom Count: 23
• Complexity: 508

Purity/Quality:

99% ^*data from raw suppliers

L-Histidine7-amido-4-methylcoumarin ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CC3=CN=CN3)N
• Isomeric SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)[C@H](CC3=CN=CN3)N
• Uses: L-Histidine 7-amido-4-methylcoumarin is an enzyme substrate. Substrates of glycosidases, phosphatases and esterases are commonly employed in microbiology, food/water, environmental and ELISA testing. Assays of peptidases are more important in clinical enzyme assays and AMC is the fluorophore employed in most of these substrates. AMC is extremely versatile since the carboxy-terminus of any amino acid or peptide can be linked to it. Most enzymes are tolerant towards the AMC structure. Typically, kinetics of enzyme reactions remain undisturbed and few inhibitory effects are observed. Due to the low basicity of the amino group, AMC is not subject to acid base equilibrium at physiological pH. Therefore, assays using AMC substrates are rather tolerant of pH.