4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione

Base Information

Chemical Name:4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione
CAS No.:1313211-51-6
Molecular Formula:C₁₁H₉N₃O₃
Molecular Weight:231.211
Hs Code.:

Synonyms:4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione

Suppliers and Price of 4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione

Supply Marketing:

Business phase:: The product has achieved commercial mass production^*data from LookChem market partment

Manufacturers and distributors:

Manufacture/Brand
Chemicals and raw materials
Packaging
price

Sigma-Aldrich
PTAD-Alkyne 97%
500mg
$ 355.00

Total 2 raw suppliers

Chemical Property of 4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione

Chemical Property:

Melting Point:176-181°C

Purity/Quality:

98%,99%, ^*data from raw suppliers

PTAD-Alkyne 97% ^*data from reagent suppliers

Safty Information:

Pictogram(s):
Hazard Codes:

MSDS Files:

Useful:

Uses Note: PTAD-Alkyne must be first activated by stirring in a 1:0.98 molar ratio with 1,3-dibromo-5,5-dimethylhydantoin (product # 157902). Activation is evident upon solution color change from colorless to deep red and the activated reagent should be used immediately. General Procedure for Protein Modification with PTAD. Part 1: PTAD activationMix together 1:0.98 molar equivalents of unactivated PTAD to 1,3-dibromo-5,5-dimethylhydantoin (product # 157902) in organic solvent (preferred solvents are DMF or acetonitrile; avoid using DMSO)Color change is observed from colorless/pale yellow to deep red (approximately 5 min of mixing).After the solution turns red, store the now activated reagent on ice and use for protein modification within 30 min. Part 2: Protein modificationAdd protein solution in mixed phosphate/Tris buffer or Tris buffer (pH should be 6 - 9) to the eppendorf tube (or other vial) containing the activated PTAD reagent prepared above and mix gently at room temperature for up to 30 min. Preferably use 10-fold molar excess of reagent relative to protein. Use protein at a minimum concentration of 1 mg/ml (higher concentrations are preferred for enhanced labeling). Remove excess unreacted PTAD by gel filtration.

Technology Process of 4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione

There total 7 articles about 4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 47.0%

: 26557-78-8
4-(prop-2-ynyloxy)aniline

: 4114-31-2
ethylhydrazine carboxylate

: 1313211-51-6
4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione

Guidance literature:: ethylhydrazine carboxylate; With 1,1'-carbonyldiimidazole; In tetrahydrofuran; at 23 ℃; for 2h;

4-(prop-2-ynyloxy)aniline; With triethylamine; In tetrahydrofuran; at 45 ℃; for 3h;
DOI:10.1002/anie.201702554

Reference yield: 45.0%

: C₁₃H₁₅N₃O₄

: 1313211-51-6
4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione

Guidance literature:: With potassium carbonate; In methanol; for 12h; Reflux;

Reference yield:

: 26557-78-8
4-(prop-2-ynyloxy)aniline

: 1313211-51-6
4-(4-(prop-2-yn-1-yloxy)-phenyl)-1,2,4-triazolidine-3,5-dione

Guidance literature:: Multi-step reaction with 2 steps

1: chloroform / 2 h / 20 °C

2: potassium carbonate / methanol / 12 h / Reflux

With potassium carbonate; In methanol; chloroform;