CCK-8

Base Information

• Chemical Name: CCK-8
• CAS No.: 72209-59-7
• Molecular Formula: C₄₉H₆₀N₁₀O₁₆S₃*2Na
• Molecular Weight: 1187.25
• Mol file: 72209-59-7.mol

Synonyms:

Chemical Property of CCK-8

Chemical Property:

Purity/Quality:

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

Useful:

Technology Process of CCK-8

There total 4 articles about CCK-8 which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 34.0%

tert-butyloxycarbonyl-β-tert-butyloxyaspartyl-O4-sulfotyrosyl-methionyl-glycyl-tryptophyl-methionyl-aspartyl-phenylalanine amide disodium salt (105567-89-3,115967-24-3) → CCK-8 (72209-59-7)

Guidance literature:

With water; mercaptoacetic acid; trifluoroacetic acid; diothiothreitol; at 0 ℃; for 3h;

Reference yield:

L-Tyr-OMe (1080-06-4) → CCK-8 (72209-59-7)

Guidance literature:

Multi-step reaction with 4 steps

1: 85 percent / DCC, hydroxybenzotriazole / dimethylformamide / 1) -10 to -5 deg C, 2 h; 2) r.t., overnight

2: 84 percent / pyridinium acetyl sulfate / dimethylformamide; pyridine / 20 h / 40 °C

3: 42 percent / ethylenediaminetetraacetic acid, dithiothreitol, papain, aq. NaOH / methanol / 0.33 h / 30 °C / pH 9.1

4: 34 percent / H₂O, CF₃CO₂H, thioglycolic acid, dithiothreitol / 3 h / 0 °C

With sodium hydroxide; ethylenediaminetetraacetic acid; water; benzotriazol-1-ol; mercaptoacetic acid; pyridinium acetyl sulfate; dicyclohexyl-carbodiimide; trifluoroacetic acid; papain; diothiothreitol; In pyridine; methanol; N,N-dimethyl-formamide;

Reference yield:

tert-butyloxycarbonyl-β-tert-butyloxyaspartyl-O4-sulfotyrosine α-methyl ester sodium salt (115967-23-2) → CCK-8 (72209-59-7)

Guidance literature:

Multi-step reaction with 2 steps

1: 42 percent / ethylenediaminetetraacetic acid, dithiothreitol, papain, aq. NaOH / methanol / 0.33 h / 30 °C / pH 9.1

2: 34 percent / H₂O, CF₃CO₂H, thioglycolic acid, dithiothreitol / 3 h / 0 °C

With sodium hydroxide; ethylenediaminetetraacetic acid; water; mercaptoacetic acid; trifluoroacetic acid; papain; diothiothreitol; In methanol;

upstream raw materials:

tert-butyloxycarbonyl-β-tert-butyloxyaspartyl-O⁴-sulfotyrosyl-methionyl-glycyl-tryptophyl-methionyl-aspartyl-phenylalanine amide disodium salt

L-Tyr-OMe

tert-butyloxycarbonyl-β-tert-butyloxyaspartyl-O⁴-sulfotyrosine α-methyl ester sodium salt

tert-butyloxycarbonyl-β-tert-butyloxyaspartyl-tyrosine α-methyl ester