Acetylene-PEG4-biotin conjugate

Base Information

• Chemical Name: Acetylene-PEG4-biotin conjugate
• CAS No.: 1458576-00-5
• Molecular Formula: C₂₁H₃₅N₃O₆S
• Molecular Weight: 457.591
• Mol file: 1458576-00-5.mol

Synonyms:

Chemical Property of Acetylene-PEG4-biotin conjugate

Chemical Property:

• Melting Point: 55-64 °C
• Boiling Point: 707.1±60.0 °C(Predicted)
• PKA: 13.90±0.40(Predicted)
• Density: 1.158±0.06 g/cm3(Predicted)
• Storage Temp.: -20°C
• Solubility.: Soluble in Water, DMSO, DCM, DMF

Purity/Quality:

97% ^*data from raw suppliers

Biotin-PEG4-alkyne 95% ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

Useful:

• Description: Biotin-PEG4-alkyne is click chemistry biotinylation reagent. The alkyne group is reactive with azide moiety via Click Chemistry reaction to form a stable triazole linkage. PEG arm increases solubility in aqueous media of the molecules conjugated to the biotin compound. It also helps to minimize steric hindrance involved with the binding to avidin molecules.
• Uses: An acetylene-biotin conjugate with PEG spacer

Technology Process of Acetylene-PEG4-biotin conjugate

There total 5 articles about Acetylene-PEG4-biotin conjugate which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

3,6,9,12-tetraoxapentadec-14-yn-1-amine (1013921-36-2,956348-61-1) + biotin (58-85-5,22879-79-4) → C21H35N3O6S (1262681-31-1,1458576-00-5)

Guidance literature:

With 1-ethyl-(3-(3-dimethylamino)propyl)-carbodiimide hydrochloride; In N,N-dimethyl-formamide; at 20 ℃; for 16h;

DOI:10.1016/j.bmc.2017.09.010

Reference yield:

3,6,9,12-tetraoxapentadec-14-yn-1-amine (1013921-36-2,956348-61-1) + (3aS,4S,6aR)-4-[5-(1H-imidazol-1-yl)-5-oxopentyl]tetrahydro-1H-thieno-[3,4-d]imidazol-2(3H)-one (101187-31-9) → C21H35N3O6S (1262681-31-1,1458576-00-5)

Guidance literature:

With N-ethyl-N,N-diisopropylamine; In N,N-dimethyl-formamide; at 25 ℃; for 12h;

Reference yield:

C19H23NO6 (1422540-93-9) → C21H35N3O6S (1262681-31-1,1458576-00-5)

Guidance literature:

Multi-step reaction with 2 steps

1: hydrazine hydrate / ethanol; water / 2 h / Reflux

2: 1-ethyl-(3-(3-dimethylamino)propyl)-carbodiimide hydrochloride / N,N-dimethyl-formamide / 16 h / 20 °C

With hydrazine hydrate; 1-ethyl-(3-(3-dimethylamino)propyl)-carbodiimide hydrochloride; In ethanol; water; N,N-dimethyl-formamide;

DOI:10.1016/j.bmc.2017.09.010

upstream raw materials:

C₁₉H₂₃NO₆

3,6,9,12-tetraoxapentadec-14-yn-1-ol

3,6,9,12-tetraoxapentadec-14-yn-1-amine

biotin