Technology Process of Nap-GFFY
There total 4 articles about Nap-GFFY which
guide to synthetic route it.
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synthetic route:
- Guidance literature:
-
With
sodium hydrogencarbonate; alkaline phosphatase;
In
aq. buffer;
pH=7.4;
Enzymatic reaction;
DOI:10.1002/cjoc.201200237
- Guidance literature:
-
Fmoc-Tyr(tBu)-OH;
With
N-ethyl-N,N-diisopropylamine;
In
dichloromethane;
at 20 ℃;
for 1h;
chlorotrityl resin
With
piperidine;
In
N,N-dimethyl-formamide;
chlorotrityl resin
C14H13NO3; N-Fmoc L-Phe;
Further stages;
DOI:10.1039/c3cc45127e
- Guidance literature:
-
Multi-step reaction with 3 steps
1: 1-ethyl-(3-(3-dimethylamino)propyl)-carbodiimide hydrochloride; N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 12 h / 20 °C
2: lithium hydroxide monohydrate / tetrahydrofuran; water / 2 h / Cooling with ice
3: N-ethyl-N,N-diisopropylamine / dichloromethane / 1 h / 20 °C
With
lithium hydroxide monohydrate; 1-ethyl-(3-(3-dimethylamino)propyl)-carbodiimide hydrochloride; N-ethyl-N,N-diisopropylamine;
In
tetrahydrofuran; dichloromethane; water; N,N-dimethyl-formamide;
DOI:10.1039/c3cc45127e