103489-33-4Relevant articles and documents
A practical molecular clip for immobilization of receptors and biomolecules on devices' surface: Synthesis, grafting protocol and analytical assay
Devouge, Sabrina,Salvagnini, Claudio,Marchand-Brynaert, Jacqueline
, p. 3252 - 3256 (2005)
Deposition of O-succinimidyl 4-(p-azido-phenyl)butanoate (4) onto inorganic device (FTIR-ATR crystal) or polymer material (filtration membrane) followed by irradiation at 254 nm led to surface functionalisation with NHS esters. Further reaction with biomolecules allowed their covalent grafting. The reactivity of the photoactivated surfaces was assayed by two methods: (i) the coupling with 3,5-bis(trifluoromethyl)benzylamine (7) and subsequent XPS analysis; (ii) the coupling with 4,5-bis-tritiated lysine (10) and subsequent LSC measurement of the radioactivity.
Synthesis and biological characterization of SQBAzide, a novel biotinylated photoaffinity probe for the study of the human platelet thromboxane A2 receptor
Halmos, Therese,Turek, Joseph W.,Le Breton, Guy C.,Antonakis, Kostas
, p. 2963 - 2968 (2007/10/03)
SQBAzide, a biotinylated, azido derivative of the TXA2 receptor antagonist, SQ31,491, was synthesized and characterized. The compound specifically inhibited human platelet aggregation mediated by TXA2 receptor activation and irreversibly labeled platelet TXA2 receptors upon exposure to ultraviolet light. This probe should prove to be of significant value for the study of the receptor-ligand binding domain.