SummaryThe complete amino acid sequence of stellacyanin, a blue copper-containing glycoprotein, is presented. The protein has been enzymatically cleaved by trypsin, chymotrypsin, and by the protease from the Staphylococcus aureus strain U8. Stellacyanin consists of a single polypeptide chain of ...detailed
We have measured the optical absorption spectra of azurin and stellacyanin in the wavelength range 1100-350 nm and in the temperature interval 290–20 K. Samples are protein aqueous solutions containing 65% (v/v) glycerol or ethylene glycol as cryoprotectants and remain homogeneous and transpare...detailed
Stellacyanin, the single blue copper protein from Rhusvernicifera, is reduced stoichiometrically by Cr(II)aq ions yielding a 1:1 adduct between the Cr(III) produced and the reduced protein. This Cr(III)-labeled stellacyanin is substitution inert and no significant loss of the label occurs during...detailed
Stellacyanin from Rhus vernificera is a blue copper protein in which the metal is coordinated to a Cys, two His and a Gln residue. We have studied the Ni(II) derivative of stellacyanin by paramagnetic 1H NMR spectroscopy. The NMR spectrum resembles that of Ni(II) Met121Gln azurin, indicating a s...detailed