Affinity labeling of stellacyanin (cas 12738-61-3) by Cr(II) aquo ions

Add time:08/28/2019    Source:sciencedirect.com

stellacyanin (cas 12738-61-3), the single blue copper protein from Rhusvernicifera, is reduced stoichiometrically by Cr(II)aq ions yielding a 1:1 adduct between the Cr(III) produced and the reduced protein. This Cr(III)-labeled stellacyanin is substitution inert and no significant loss of the label occurs during extensive dialysis for more than a week. Oxidation by O2 of the Cr(III)-labeled Cu(I) stellacyanin does not cause the loss of Cr(III) either. Furthermore, reduction of the Cr(III)-labeled stellacyanin Cu(II) by a second equivalent of Cr(II) may be attained without any further labeling. Thus, the one mole of Cr ions binds to stellacyanin during the first reduction step and is most probably coordinated at a specific locus on that protein.

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