177966-52-8 Usage
General Description
5-Bromo-4-chloro-3-indolyl beta-D-cellobioside is a chemical compound that is commonly used in the study of enzyme substrates and as a sensitive chromogenic substrate for the detection of various enzymes such as cellulases and beta-glucosidases. It is a substrate for the detection of the activity of beta-glucosidases, which catalyze the hydrolysis of cellobiose to form glucose. The compound is often used in the field of biotechnology and biochemistry to study the activity and specificity of enzymes, as well as in the development of enzyme assays and detection methods. It is a valuable tool in the investigation of the enzymatic breakdown of cellulose and related carbohydrate polymers, with potential applications in the development of biofuels and bioremediation.
Check Digit Verification of cas no
The CAS Registry Mumber 177966-52-8 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,7,7,9,6 and 6 respectively; the second part has 2 digits, 5 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 177966-52:
(8*1)+(7*7)+(6*7)+(5*9)+(4*6)+(3*6)+(2*5)+(1*2)=198
198 % 10 = 8
So 177966-52-8 is a valid CAS Registry Number.
177966-52-8Relevant articles and documents
Indoxylic acid esters as convenient intermediates towards indoxyl glycosides
Boettcher, Stephan,Thiem, Joachim
, p. 564 - 574 (2014/02/14)
Indoxylic acid methyl and allyl esters with varied halide-substitution patterns were obtained in excellent yields using a scalable route. Phase-transfer glycosylation of these key intermediates was carried out with various glycosyl halides. Subsequent mild silver-mediated decarboxylation followed by Zemplen deacetylation led to indoxyl glycosides in good overall yields. Indoxyl glycosides are well-established and widely used tools for enzyme screening and enzyme-activity monitoring. In the past, their synthesis has been difficult, so this new approach has led to a variety of useful structures. Indoxyl glycosides with varied halide-substitution patterns were synthesized using indoxylic acid esters as key intermediates. Glycosylation under phase-transfer conditions, ester cleavage, and mild decarboxylation led to the indoxyl glycosides in good yields. This approach enables access to a number of different indoxyl compounds. Copyright
