1969-54-6Relevant articles and documents
2-MERCAPTOBENZOTHIAZOLE-AN IMPROVED REAGENT FOR THE REMOVAL OF METHYL PHOSPHATE PROTECTING GROUPS FROM OLIGODEOXYNUCLEOTIDE PHOSPHOTRIESTERS.
Andrus, Alex,Beaucage, Serge L.
, p. 5479 - 5482 (1988)
An equimolar (1.5 M) solution of 2-mercaptobenzothiazole and N,N-diisopropylethylamine in 1-methyl-2-pyrrolidinone efficiently removed the methyl phosphate protecting groups from deoxynucleotide phosphotriesters and yielded synthetic oligomers of high genetic integrity.This odorless reagent can therefore substitute for hazardous thiophenol usually used for this purpose.
Ester exchange reaction of oxyphosphorane with thymidine
Chen,Zhao
, p. 3691 - 3694 (1995)
Ester exchange reaction of oxyphosphorane (1) with thymidine (2) afforded diastereomeric spirooxyphosphoranes 3 under mild conditions in good yield. After hydrolysis, thymidine phosphates monomers (5,6) and dimer (7) were obtained.
Large-scale oligonucleotide synthesis by the H-phosphonate method
Gaffney,Jones
, p. 2619 - 2622 (2007/10/02)
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