19916-77-9Relevant articles and documents
Synthesis of oligodeoxynucleotides containing 6-N-([13C]methyl)adenine and 2-N-([13C]methyl)guanine
Hofmann, Mechtild,Acedo, Montse,Fagan, Patricia,Wemmer, David,Eritja, Ramon,Diaz, Antonio R.
, p. 1825 - 1828 (1997)
Oligonucleotides containing 6-N-([l3C]methyl)adenine and 2-N-([13C]methyl)guanine have been prepared for NMR studies using the deprotection step to introduce the [13C]methylamine group. For this purpose, the use of 2′-deoxy-6-O-(pentafluorophenyl)inosine 1 and 2′-deoxy-2-fluoro-6-O-[2-(4-nitrophenyl)-ethyl]inosine 2 as precursors of the N-methylated nucleosides is described. Preliminary NMR characterization of the 13C-labelled oligonucleotides shows that the 13C chemical shift of the methyl group in N-methylguanine is sensitive to duplex formation, making it a useful local probe.