100817-30-9Relevant articles and documents
FORMATION OF 6-O-α-MALTOSYLCYCLOMALTO-OLIGOSACCHARIDES FROM α-MALTOSYL FLUORIDE AND CYCLOMALTO-OLIGOSACCHARIDES BY PULLULANASE
Kitahata, Sumio,Yoshimura, Yoshimichi,Okada, Shigetaka
, p. 303 - 314 (2007/10/02)
Branched cyclomalto-oligosaccharides are formed when pullulanase from Bacillus acidopullulyticus is incubated with α-maltosyl fluoride (α-G2F) as substrate in the presence of cyclomalto-oligosaccharides as acceptor.It was shown by enzymic and chemical methods that the structures of these branched cyclomaltooligosaccharides are 6-O-α-maltosylcyclomalt-ohexaose (G2-cG6), 6,6'-di-O-α-maltosylcyclomaltohexaose , 6-O-α-maltosylcyclomaltoheptaose (G2-cG7), 6,6'-di-O-α-maltosylcyclomaltoheptaose , and 6,6',6''-tri-O-α-maltosylcyclomaltoheptaose .The optimal conditions for formation of G2-cG6 were studied.About 12 mM G2-cG6 was produced when pullulanase (2.8 U/mL) was incubated with 40 mM α-G2F as substrate and 90 mM cyclomaltohexaose (cG6) as acceptor for 1 h at 60 deg C.The yield of G2-cG6, the main transfer-product, was twenty times that by the condensation of cG6 with α-maltose in the presence of pullulanase.