10215-75-5Relevant articles and documents
Derivative of 17-hydroxysteroid, detection reagent and preparation method thereof
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Paragraph 0071; 0072; 0073; 0074; 0075, (2019/08/20)
The invention discloses a derivative of 17-hydroxysteroid, a detection reagent and a preparation method thereof, and relates to the technical field of biological detection. A 17-hydroxysteroid immunogen prepared by using the 17-hydroxysteroid derivative has high immunogenicity, and antibody specificity is strong and titer is high. A 17-hydroxyl steroid conjugate in a homogeneous enzyme immunoassayreagent prepared by using the derivative is linked to genetically engineered recombinant glucose-6-phosphate dehydrogenase. Thereby, detection sensitivity is significantly enhanced, and a sample withthe concentration being as low as 80 nmol/L can be detected effectively; and the detection reagent has strong specificity and has no cross reaction with common 92 interferents. High-throughput and rapid detection of the 17-hydroxysteroid content can be realized by a fully automatic biochemical analyser. The detection result is stable, accuracy is high, and the detection method is simple and is easy for implementation and promotion and application.