1031702-80-3Relevant articles and documents
Dextran based photodegradable hydrogels formed via a Michael addition
Peng, Ke,Tomatsu, Itsuro,Van Den Broek, Bram,Cui, Chao,Korobko, Alexander V.,Van Noort, John,Meijer, Annemarie H.,Spaink, Herman P.,Kros, Alexander
, p. 4881 - 4887 (2011)
A photodegradable, covalently crosslinked hydrogel system has been constructed from the biocompatible polymers dextran and poly(ethylene glycol) using the acrylate-thiol Michael addition as the crosslinking method. Light sensitivity of the hydrogel was in
Synthesis and biological evaluation of a novel photo-activated histone deacetylase inhibitor
Dear, Anthony E.,Liu, Hongbin,Mountford, Simon,Robinson, Andrea,Sama, Gopal R.,Thompson, Phillip
, (2020)
Hydroxamic acid-based histone deacetylase inhibitors (HDACi) are a class of epigenetic agents with potentially broad therapeutic application to several disease states including post angioplasty mediated neointimal hyperplasia (NIH). Precise spatiotemporal
Near infrared light triggered release of biomacromolecules from hydrogels loaded with upconversion nanoparticles
Yan, Bin,Boyer, John-Christopher,Habault, Damien,Branda, Neil R.,Zhao, Yue
, p. 16558 - 16561 (2012)
Using a photosensitive hybrid hydrogel loaded with upconversion nanoparticles (UCNPs), we show that continuous-wave near-infrared (NIR) light (980 nm) can be used to induce the gel-sol transition and release large, inactive biomacromolecules (protein and
PEG-PEI-modified gated N-doped mesoporous carbon nanospheres for pH/NIR light-triggered drug release and cancer phototherapy
Panda, Snigdharani,Bhol, Chandra Sekhar,Bhutia, Sujit Kumar,Mohapatra, Sasmita
, p. 3666 - 3676 (2021/05/17)
A novel hybrid drug carrier has been designed, taking N-doped mesoporous carbon (NMCS) as the core and PEG-PEI as the outer shell. NMCS was functionalized with a photocleavable nitrobenzyl-based linker following a click reaction. Gemcitabine was loaded in
Photoproximity Profiling of Protein-Protein Interactions in Cells
Carlos, Anthony,Lee, Gihoon,McCutcheon, David C.,Moellering, Raymond E.,Montgomery, Jeffrey E.
, p. 146 - 153 (2020/01/31)
We report a novel photoproximity protein interaction (PhotoPPI) profiling method to map protein-protein interactions in vitro and in live cells. This approach utilizes a bioorthogonal, multifunctional chemical probe that can be targeted to a genetically encoded protein of interest (POI) through a modular SNAP-Tag/benzylguanine covalent interaction. A first generation photoproximity probe, PP1, responds to 365 nm light to simultaneously cleave a central nitroveratryl linker and a peripheral diazirine group, resulting in diffusion of a highly reactive carbene nucleophile away from the POI. We demonstrate facile probe loading, and subsequent interaction- A nd light-dependent proximal labeling of a model protein-protein interaction (PPI) in vitro. Integration of the PhotoPPI workflow with quantitative LC-MS/MS enabled unbiased interaction mapping for the redox regulated sensor protein, KEAP1, for the first time in live cells. We validated known and novel interactions between KEAP1 and the proteins PGAM5 and HK2, among others, under basal cellular conditions. By contrast, comparison of PhotoPPI profiles in cells experiencing metabolic or redox stress confirmed that KEAP1 sheds many basal interactions and becomes associated with known lysosomal trafficking and proteolytic proteins like SQSTM1, CTSD, and LGMN. Together, these data establish PhotoPPI as a method capable of tracking the dynamic subcellular and protein interaction "social network" of a redox-sensitive protein in cells with high temporal resolution.
