1032047-31-6Relevant articles and documents
A two-step sulfurization for efficient solution-phase synthesis of phosphorothioate oligonucleotides
Ghisaidoobe, Amar B.T.,de Koning, Martijn C.,Duynstee, Howard I.,Ten Kortenaar, Paul B.W.,Overkleeft, Herman S.,Filippov, Dmitri V.,van der Marel, Gijs A.
, p. 3129 - 3132 (2008/09/20)
A solution-phase approach for the synthesis of phosphorothioate oligonucleotides that circumvents the use of chromatographic purifications of protected phosphorothioate intermediates was developed. Implementation of a two-step sulfurization protocol in the phosphoramidite method allows efficient isolation of the intermediate phosphorothioates by extractions. The viability of this approach is demonstrated by the synthesis of a hexameric phosphorothioate oligonucleotide fragment.
