913697-73-1Relevant academic research and scientific papers
Simple and efficient solution-phase synthesis of oligonucleotides using extractive work-up
De Koning, Martijn C.,Ghisaidoobe, Amar B. T.,Duynstee, Howard I.,Ten Kortenaar, Paul B. W.,Filippov, Dmitri V.,Van Der Marel, Gijs A.
, p. 1238 - 1245 (2006)
A solution-phase synthesis protocol amenable to scale-up was developed for the preparation of oligonucleotides employing phosphoramidite chemistry and DMTr/iBu/Bz-protected monomers. Isolation of intermediates was accomplished by means of extractions as the only purification tool. The potential of the method is demonstrated with the synthesis of a hexameric DNA fragment in high yield and purity.
