10326-73-5Relevant articles and documents
PREPARATION OF D-GLUCOSE AND D-GLUCOSE
Malaisse-Lagae, F.,Zaehner, D.,Sener, A.,Malaisse, W. J.
, p. 159 - 165 (2007/10/02)
An enzymic method is presented for the preparation of 14C-labelled and C1-deuterated D-glucose, to be used as a radioactive tracer in metabolic studies dealing with the fate of D-glucose.The procedure takes advantage of the deuteration of 14C-labelled D-fructose 6-phosphate during exposure to phosphomannoisomerase in D2O.It further includes the conversion of the deuterated hexose monophosphates to D-fructose 1,6-bisphosphate, its isolation and hydrolysis to D-fructose 6-phosphate and the partial conversion of the latter ester to D-glucose 6-phosphate, which is then isolated by ion exchange chromatography and hydrolyzed to unesterified D-glucose.The extent of deuteration on the C1 of D-glucose was judged, after conversion to D-glucose 6-phosphate, from the isotopic discrimination in reaction velocity exerted towards the C1-deuterated ester by glucose-6-phosphate dehydrogenase.The degree of isotopic discrimination was virtually identical to that found with D-glucose 6-phosphate prepared from pure D-glucose.