1032678-07-1Relevant articles and documents
Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide
Andina, Diana,Brambilla, Davide,Munzinger, Noah,Frei, Jasmine,Zivko, Cristina,Leroux, Jean-Christophe,Luciani, Paola
, p. 4765 - 4769 (2017)
Extracellular detection of endogeneous analytes (e.g., superoxide) can provide important insights into mechanisms of homeostasis and diseases, such as tumorigenesis. A ratiometric probe with a fluorescent reference and an analyte-specific switch-on dye was developed. Detection of ROS in the extracellular milieu was ensured by connecting the two fluorophores with a modular peptide-nucleic-acid-based linker. The ROS-sensing ability was assessed and validated in cell-free assays and in cell culture.
Small Molecule Recognition Triggers Secondary and Tertiary Interactions in DNA Folding and Hammerhead Ribozyme Catalysis
Mao, Jie,Desantis, Chris,Bong, Dennis
, p. 9815 - 9818 (2017)
We have identified tris(2-aminoethyl)amine (tren)-derived scaffolds with two (t2M) or four (t4M) melamine rings that can target oligo T/U domains in DNA/RNA. Unstructured T-rich DNAs cooperatively fold with the tren derivatives to form hairpin-like structures. Both t2M and t4M act as functional switches in a family of hammerhead ribozymes deactivated by stem or loop replacement with a U-rich sequence. Catalysis of bond scission in these hammerhead ribozymes could be restored by putative t2M/t4M refolding of stem secondary structure or tertiary bridging interactions between loop and stem. The simplicity of the t2M/t4M binding site enables programming of allostery in RNAs, recoding oligo-U domains as potential sites for secondary structure or tertiary contact. In combination with a facile and general method for installation of the t2M motif on primary amines, the method described herein streamlines design of synthetic allosteric riboswitches and small molecule-nucleic acid complexes.
Storable arylpalladium(II) reagents for alkene labeling in aqueous media
Simmons, Rebecca L.,Yu, Robert T.,Myers, Andrew G.
, p. 15870 - 15873 (2011/11/13)
We show that arylpalladium(II) reagents linked to biotin and indocyanine dye residues can be prepared by decarboxylative palladation of appropriately substituted electron-rich benzoic acid derivatives. When prepared under the conditions described, these organometallic intermediates are tolerant of air and water, can be stored for several months in solution in dimethyl sulfoxide, and permit biotin- and indocyanine dye-labeling of functionally complex olefinic substrates in water by Heck-type coupling reactions.
A convenient synthesis of cyanine dyes: Reagents for the labeling of biomolecules
Kvach, Maksim V.,Ustinov, Alexey V.,Stepanova, Irina A.,Malakhov, Andrei D.,Skorobogatyi, Mikhail V.,Shmanai, Vadim V.,Korshun, Vladimir A.
experimental part, p. 2107 - 2117 (2009/04/05)
Four tetramethylindo(di)carbocyanine-derived carboxylic acids have been prepared using a modified one-pot procedure for the dye assembly. The acids were converted into oxysuccinimide esters and phosphoramidite reagents. The efficiency of the reagents in t