1045786-06-8

Upstream product

• 1045786-04-6 N-(benzyloxycarbonyl) [4,6-di-O-acetyl-2-azido-2-deoxy-3-O-(3,4,6-tri-O-acetyl-2-O-(2,5-difluorobenzoyl)-β-D-galactopyranosyl)-(1->3)-α-D-galactopyranosyl]-L-serine t-butyl ester 
• 507-09-5 tiolacetic acid 

Relevant academic research and scientific papers

Chemo-enzymatic synthesis of C-9 acetylated sialosides

A chemo-enzymatic synthesis of [(5-acetamido-9-O-acetyl-3,5-dideoxy-d-glycero-α-d-galacto-2-nonulopyranosylonic acid)-(2→3)-O-(β-d-galactopyranosyl)-(1→3)-O-(2-acetamido-2-deoxy-α-d-galactopyranosyl)]-l-serine acetate (1) has been accomplished by a regioselective chemical acetylation of Neu5Ac (2) to give 9-O-acetylated sialic acid 3, which was enzymatically converted into CMP-Neu5,9Ac2 (4) employing a recombinant CMP-sialic acid synthetase from Neisseria meningitis [EC 2.7.7.43]. The resulting compound was then employed for the enzymatic glycosylation of the C-3′ hydroxyl of chemically prepared glycosylated amino acid 10 using recombinant rat α-(2→3)-O-sialyltransferase expressed in Spodooptera frugiperda [EC 2.4.99.4] to give, after deprotection of the Nα-benzyloxycarbonyl (CBz)-protecting group of serine, target compound 1. The Nα-CBz-protected intermediate 11 can be employed for the synthesis of glycolipopeptides for immunization purposes.