104655-84-7Relevant academic research and scientific papers
SOLID-PHASE SYNTHESIS OF MODIFIED OLIGODEOXYRIBONUCLEOTIDES WITH AN ACRIDINE DERIVATIVE OR A THIOPHOSPHATE GROUP AT THEIR 3' END
Asseline, U.,Thuong, Nguyen T.
, p. 2521 - 2524 (1989)
Use of a derivatized support involving the 2,2'-diethyldithio-group 7 allows the automated synthesis of oligodeoxyribonucleotide bearing acridine derivative (via nucleoside -3'-acridinylphosphoramidite 3) or 3'phosphorothioate group (including the sulfuri
Solid-Phase Synthesis, Hybridizing Ability, Uptake, and Nuclease Resistant Profiles of Position-Selective Cationic and Hydrophobic Phosphotriester Oligonucleotides
Monfregola, Luca,Caruthers, Marvin H.
, p. 9147 - 9158 (2015)
Analogues of oligonucleotides and mononucleotides with hydrophobic and/or cationic phophotriester functionalities often generate an improvement in target affinity and cellular uptake. Here we report the synthesis of phosphotriester oligodeoxyribonucleotid
An improved method for the preparation of 4-cyano-2-butenyl- deoxyribonucleosidephosphoramidites
Duckworth,Ackroyd
, p. 1227 - 1229 (1999)
An improved alternative synthesis of 4-cyano-2-butenyldeoxy nucleosidephosphoramidites in >100g quantities is described via reaction of the phosphordiamidites with 4-cyano-2-buten-1-ol.
Syntheses of prodrug-type phosphotriester oligonucleotides responsive to intracellular reducing environment for improvement of cell membrane permeability and nuclease resistance
Hayashi, Junsuke,Samezawa, Yusuke,Ochi, Yosuke,Wada, Shun-ichi,Urata, Hidehito
, p. 3135 - 3138 (2017)
We synthesized prodrug-type phosphotriester (PTE) oligonucleotides containing the six-membered cyclic disulfide moiety by using phosphoramidite chemistry. Prodrug-type oligonucleotides named “Reducing-Environment-Dependent Uncatalyzed Chemical Transforming (REDUCT) PTE oligonucleotides” were converted into natural oligonucleotides under cytosol-mimetic reductive condition. Furthermore, the REDUCT PTE oligonucleotides were robust to nuclease digestion and exhibited good cell membrane permeability.
Compound, fluorescence-labeled-introducing agent, and introducing fluorescent labeling method (by machine translation)
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Paragraph 0098, (2020/01/07)
[Problem] no fluorescence whereas the extracellular, intracellular fluorescence of fluorescently labeled compound is incorporated, and such fluorescent labeling for introducing fluorescent labeling agent is introduced. (1) A compound represented by general formula [a] is used. In the general formula (1), the A, biological material or derivatives thereof may be incorporated into the cell through a cell membrane structure 1 comprises a divalent group, the FL, fluorescent light having a fluorescent unit which exhibits cyclic backbone nitrogen atom, L is, a monovalent group FL A coupling 2, the X, a single bond or O - (=O) a - C, wavy lines represent bonds, the nitrogen atom is bound to a FL included, the Ar, para or ortho position of the aromatic ring which has a valence bond 2, R is, nitro or - S e S a-R1 And, R1 Is, a monovalent group, n the, an integer of 1 - 3. [Drawing] no (by machine translation)
A prodrug compound, a prodrug-oligonucleotide synthesis reagents, and manufacture of oligonucleotide-prodrug compounds
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Paragraph 0069; 0070, (2017/02/24)
PROBLEM TO BE SOLVED: To provide a new prodrug compound where a protective group capable of leaving in a cell is added to an OH group or an SH group of a phosphate group included in a synthetic oligonucleotide which may be a phosphorothioate-type analog o
PHOSPHORAMIDITE BUILDING BLOCKS FOR SUGAR-CONJUGATED OLIGONUCLEOTIDES
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Paragraph 0172, (2016/04/19)
Novel nucleoside phosphoramidite building blocks for preparation of synthetic oligonucleotides containing at least one phosphotriester linkage conjugated to a monosaccharide and synthetic processes for making the same are disclosed. Furthermore, oligomeri
Synthesis of novel cationic spermine-conjugated phosphotriester oligonucleotide for improvement of cell membrane permeability
Hayashi, Junsuke,Hamada, Tomoko,Sasaki, Ikumi,Nakagawa, Osamu,Wada, Shun-Ichi,Urata, Hidehito
, p. 3610 - 3615 (2015/08/11)
A spermine-conjugated ethyl phosphotriester oligonucleotide was obtained by solid-phase synthesis based on phosphoramidite chemistry. The ethyl phosphotriester linkage was robust to exonuclease digestion and stable in fetal bovine serum. Cell membrane permeability of the spermine-conjugated ethyl phosphotriester oligonucleotide was studied by fluorescence experiments. The effective cell penetrating potency of the spermine-conjugated ethyl phosphotriester oligonucleotide was determined by confocal laser scanning microscopy and measurement of intracellular fluorescence intensity.
PHOSPHOROUS PROTECTING GROUPS AND METHODS OF PREPARATION AND USE THEREOF
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Paragraph 00133; 00134, (2015/11/23)
Aspects of the present disclosure include compositions that make use of phosphorus and/or nucleobase protecting groups which find use in the synthesis of long polynucleotides. Phosphorus protecting groups are provided that help increase the stepwise coupl
The design, synthesis and evaluation of hypoxia-activated pro-oligonucleotides
Zhang, Nan,Tan, Chunyan,Cai, Puqin,Zhang, Peizhuo,Zhao, Yufen,Jiang, Yuyang
supporting information; experimental part, p. 3216 - 3218 (2009/12/01)
Hypoxia-activated pro-oligonucleotides were synthesized through the commercial phosphoramidite method, and could be readily cleaved to form normal oligos with good hypoxia selectivity in vitro under the effect of reductases, as well as in tumor cell extra
