1051916-41-6Relevant articles and documents
SERRS-based enzymatic probes for the detection of protease activity
Ingram, Andrew,Byers, Louise,Faulds, Karen,Moore, Barry D.,Graham, Duncan
supporting information; experimental part, p. 11846 - 11847 (2009/02/05)
Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be switched off and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml-1. Copyright