1052-38-6

Basic information

• Product Name: BISMARCK BROWN Y
• Synonyms: PHENYLENE BROWN;VESUVINE G;VESUVINE;VESUVIN;MANCHESTER BROWN;BISMARCK BROWN Y (G);BISMARCK BROWN Y;BISMARK BROWN Y
• CAS NO: 1052-38-6
• Molecular Formula: C₁₈H₁₈N₈
• Molecular Weight: 419.31
• EINECS: 233-314-3
• Product Categories: Dyes and Pigments;Solvent Dyestuff
• Mol File: 1052-38-6.mol
• Article Data: 0

Chemical Properties

• Melting Point: 222 C (dec.)
• Boiling Point: 696.424 °C at 760 mmHg
• Flash Point: 374.984 °C
• Appearance: /
• Density: 1.427 g/cm³
• Vapor Pressure: 3.04E-19mmHg at 25°C
• Refractive Index: 1.738
• PKA: 3.34±0.10(Predicted)
• Water Solubility: 18μg/L at 20℃
• CAS DataBase Reference: BISMARCK BROWN Y(CAS DataBase Reference)
• NIST Chemistry Reference: BISMARCK BROWN Y(1052-38-6)
• EPA Substance Registry System: BISMARCK BROWN Y(1052-38-6)

Safety Data

• Hazard Codes: Xn
• Statements: 20/21/22-36/37/38
• Safety Statements: 22-24/25-36-26
• WGK Germany: 3
• Hazardous Substances Data: 1052-38-6(Hazardous Substances Data)

Usage

Description

BISMARCK BROWN Y is a bis(azo) compound that is 1,1'-(1,3-phenylene)bis(diazene) in which both azene hydrogens are replaced by 2,4-diaminophenyl groups. It is a metachromatic dye which stains acid mucins yellow and is also a constituent of Papanicolaou's EA solutions, used for cervical and other smears. Its dihydrochloride salt is also a biological dye known as Bismark brown Y. It is characterized by its yellow brown color and is soluble in ethanol, acetone, and benzene. It exhibits different color changes when interacting with concentrated sulfuric and nitric acids, and its dye aqueous solution does not change color when hydrochloric acid is added, but it forms an orange precipitation when 10% sodium hydroxide solution is added.

Uses

Used in Histopathology:
BISMARCK BROWN Y is used as a staining agent for histopathological applications, particularly for staining acid mucins in tissue samples. Its metachromatic properties allow for the clear visualization and differentiation of various cellular structures, making it a valuable tool in the diagnosis of various conditions.
Used in Cytology:
In cytology, BISMARCK BROWN Y is used as a constituent of Papanicolaou's EA solutions for staining cervical and other smears. Its ability to stain acid mucins yellow helps in the identification and analysis of cellular abnormalities, contributing to the early detection and diagnosis of various diseases, including cancer.
Used in Research and Diagnostics:
BISMARCK BROWN Y is also used as a biological dye in research and diagnostic applications, where its unique staining properties can be utilized to study and identify specific cellular components and structures. Its versatility in staining and solubility in various solvents make it a valuable tool in both laboratory and clinical settings.

Preparation

2 Moore’s Nitrous acid?treatment 3 Moore’s Benzene-1,3-diamine dihydrochloride (this product may be based on the double azo dyes as the main component of the mixture, and commodity dye is usually composed of dihydrochloride composition), and made free base.

Flammability and Explosibility

Nonflammable

InChI:InChI=1/C18H18N8/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22/h1-10H,19-22H2

Synthetic route

m-phenylenediamine (108-45-2) + diazotized m-phenylenediamine → m-bis(2,4-diaminophenylazo)benzene (1052-38-6)

Conditions	Yield
With water; sodium carbonate der abfiltrierte und gewaschene Niederschlag wird mit schwacher Sodaloesung gekocht; man loest ihn dann in warmer verd.Salzsaeure, faellt die kalt filtrierte Loesung durch Ammoniak und behandelt den Niederschlag mit Benzol;

Upstream product

• 108-45-2 m-phenylenediamine