106562-32-7

Usage

Uses

Used in Fluorescent Labeling:
AMCA is used as a fluorescent protein labeling agent for various applications, including multiplex immunophenotyping. Its N-hydroxysuccinimide ester reacts with lysine residues to form stable amide links, and upon activation with UV light, it displays emission maxima of 400-460 nm.
Used in Contrasting Probes:
In the field of microscopy and imaging, 7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is used as contrasting probes for double and triple labeling in immunofluorescence assays. Its blue fluorescence allows for clear differentiation between multiple markers, enhancing the accuracy and efficiency of these assays.
Used in Non-Activated, Amine-Reactive Fluorescent Probes:
AMCA is also utilized as a non-activated, amine-reactive fluorescent probe in various research and diagnostic applications. Its ability to form stable amide links with lysine residues makes it a valuable tool for detecting and analyzing proteins and other biomolecules.

InChI:InChI=1/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)

Upstream product

• 7159-96-8 ethyl N-(3-hydroxyphenyl)carbamate 
• 1190984-61-2 7-(N-acetyl-[(L)-aspartyl]-[(L)-glutamyl]-[(L)-valinyl]-[(L)-aspartyl])-amino-4-methyl-3-coumarinylacetic amide 
• 591-27-5 m-Hydroxyaniline 
• 113721-84-9 7-carboethoxyamido-4-methylcoumarin-3-acetic acid ethyl ester 

Downstream Products

• 207118-52-3 {7-[3-(4-Cyano-phenyl)-thioureido]-4-methyl-2-oxo-2H-chromen-3-yl}-acetic acid 
• 207118-56-7 [2-(2-{7-[3-(4-Cyano-phenyl)-thioureido]-4-methyl-2-oxo-2H-chromen-3-yl}-acetylamino)-ethyl]-carbamic acid tert-butyl ester 
• 207118-58-9 [2-(2-{7-[3-(4-Cyano-phenyl)-2-methyl-isothioureido]-4-methyl-2-oxo-2H-chromen-3-yl}-acetylamino)-ethyl]-carbamic acid tert-butyl ester 
• 1202357-02-5 methyl 7-(2,4-dinitrophenylsulfonamido)-4-methylcoumarin-3-acetate 
• 1202357-03-6 7-(2,4-dinitrophenylsulfonamido)-4-methylcoumarin-3-acetic acid 
• 1282033-98-0 methyl 7-(2-cyano-4-nitrobenzensulfonamido)-4-methylcoumarin-3-acetate 
• 1282033-99-1 7-(2-cyano-4-nitrobenzensulfonamido)-4-methyl-3-coumarinylacetic acid 

Relevant academic research and scientific papers

Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation

Enzyme activity in live cells is dynamically regulated by small-molecule transmitters for maintaining normal physiological functions. A few probes have been devised to measure intracellular enzyme activities by fluorescent imaging, but the study of the re

A labeled guanidine ligand for studying sweet taste

A synthesis of a biotinylated-, coumarin-substituted-N,N'-diarylguanidin 1 is reported. This ligand has structural features conducive to studying sweet taste including a fluorescent tag to facilitate spectroscopic studies of binding to protein-receptors f

7-amino-4-methyl-coumarin-3-carboxyalkyl derivates and fluorescent conjugates thereof

A novel compound having the formula (I): STR1 or a reactive derivative or functional equivalent thereof, wherein m is an integer from 1 to 4, and R1 and R2 are same or different and selected from hydrogen and 1-4C alkyl; a fluorescent conjugate having the formula (III): STR2 wherein m R1 and R2 have the meaning given in claim 1, and R4 is a substituent attached to the keto group of formula (III) by a covalen bond. The new compounds and conjugates are usefual as fluorescent labelling agents.