107832-32-6Relevant articles and documents
Esterase screening using whole cells of Brazilian soil microorganisms
Mantovani, Simone M.,De Oliveira, Luciana G.,Marsaioli, Anita J.
, p. 1484 - 1489 (2010)
A miniaturized enzymatic assay using fluorescent probes to reveal esterase producing microorganisms was optimized and applied to screen 64 soil bacterial strains. The best results were validated using traditional non-fluorogenic assays with acetyl and propanoyl phenylethanol to confirm the miniaturized results. The most active microorganisms belong to the genus Bacillus showing esterase activity and good enantiomeric ratios for the resolution of phenylethanol derivatives (E > 30). Part of the microorganisms are kept in our laboratory in glycerol or freezedried and the best microorganisms will be deposited in the CBMAI/CPQBA/Unicamp culture collection.
Continuous green biocatalytic processes using ionic liquids and supercritical carbon dioxide
Lozano, Pedro,De Diego, Teresa,Carrie, Daniel,Vaultier, Michel,Iborra, Jose L.
, p. 692 - 693 (2002)
Soluble Candida antarctica lipase B dissolved in ionic liquids showed good synthetic activity, enantioselectivity and operational stability in supercritical carbon dioxide for both butyl butyrate synthesis and the kinetic resolution of 1-phenylethanol processes by transesterification.
Supported Ionic Liquid-Like Phases (SILLPs) for enzymatic processes: Continuous KR and DKR in SILLP-scCO2 systems
Lozano, Pedro,Garcia-Verdugo, Eduardo,Karbass, Naima,Montague, Kimberley,De Diego, Teresa,Burguete, M. Isabel,Luis, Santiago V.
, p. 1803 - 1810 (2010)
The immobilisation of Candida antarctica lipase B (CALB) onto SILLPs has been studied in order to obtain the maximum activity, selectivity and stability of the resulting supported biocatalyst for the KR of rac-1-phenylethanol by flow processes using scCO2. This involves the use of hydrophilic SILLPs (chloride anion) with high loadings of IL moieties and CALB. The combination of the immobilized biocatalyst with an acidic zeolite has allowed us to carry out DKR processes. Excellent results in terms of yield (92%) and enantioselectivity (>99.9% ee) are obtained by the use of a one-pot reactor containing a mixture of both catalyst particles under scCO2 flow conditions (50 °C, 10 MPa), resulting in an environmentally friendly process with high productivity and enantioselectivity.
18O-Labeled chiral compounds enable the facile determination of enantioselectivity by mass spectroscopy
Mandai, Kyoko,Tsuchihashi, Yuta,Ashikari, Yosuke,Yoshida, Jun-ichi,Nagaki, Aiichiro
, (2020)
The synthesis of 18O-labeled enantioenriched compounds and their facile evaluation system to determine enantiomeric excess (ee) using mass spectroscopy was described. Equimolar mixture of 18O-labeled and non-labeled pseudo-enantiomers were used as a substrate for enzyme-catalyzed kinetic resolution. Ees determined by mass spectroscopy showed good agreement with those by HPLC. Our method would be a promising tool for fast evaluation of ee and contribute to development of enantioselective transformations.
Macroporous polymers tailored as supports for large biomolecules: Ionic liquids as porogenic solvents and as surface modifiers
Izquierdo, Diana Flor,Yates, Malcolm,Lozano, Pedro,Burguete, M. Isabel,García-Verdugo, Eduardo,Luis
, p. 20 - 27 (2014)
Highly ordered rod-like polymeric monoliths with large-pores have been successfully synthesized using ionic liquids (ILs) IL-1 (1-Butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [BMIM][NTf2]) and IL-2 (1-octyl-3-methylimidazolium bi
An efficient microwave-assisted enzymatic resolution of alcohols using a lipase immobilised on supported ionic liquid-like phases (SILLPs)
Izquierdo, Diana F.,Bernal, Juana M.,Burguete, M. Isabel,Garcia-Verdugo, Eduardo,Lozano, Pedro,Luis, Santiago V.
, p. 13123 - 13126 (2013)
The combined action of microwave irradiation and CALB immobilised onto polymeric SILLPs leads to an excellent activity improvement (28 fold) and operational stability towards reuse (12 operation cycles). This procedure also allows the microwave-assisted one-pot DKR by the combination with a solid acid catalyst.
Benzotetramisole: A remarkably enantioselective acyl transfer catalyst
Birman, Vladimir B.,Li, Ximin
, p. 1351 - 1354 (2006)
A commercially available pharmaceutical, tetramisole, was found to be a competent enantioselective acylation catalyst. Its benzannellated analogue, benzotetramisole (BTM), produced outstanding enantioselectivities in kinetic resolution of secondary benzylic alcohols.
Immobilized redox enzymatic catalysts: Baeyer-Villiger monooxygenases supported on polyphosphazenes
Cuetos, Anibal,Rioz-Martinez, Ana,Valenzuela, Maria L.,Lavandera, Ivan,De Gonzalo, Gonzalo,Carriedo, Gabino A.,Gotor, Vicente
, p. 178 - 183 (2012)
A novel method has been employed for the selective covalent co-immobilization of a Baeyer-Villiger monooxygenase (phenylacetone monooxygenase from Thermobifida fusca) and a NADPH recycling enzyme (glucose-6-phosphate dehydrogenase) on the same polyphosphazene carrier for the first time starting from {NP[O2C12H8-x(NH 2)x]}n (x ranging from 0.5 to 2) using glutaraldehyde as connector. In all cases the preparation was active and it was found that the optimum proportion of amino groups in the starting polyphosphazene was 0.5 per monomer. The immobilized biocatalysts showed similar selectivity when compared with the isolated monooxygenase, demonstrating the potential of this novel type of immobilizing material, although their recyclability must still be improved.