108409-83-2

Basic information

• Product Name: FH 535
• Synonyms: FH 535;N-(2-Methyl-4-nitrophenyl)-2,5-dichlorobenzenesulfonaMide;2,5-Dichloro-N-(2-Methyl-4-nitrophenyl)benzenesulfonaMide;2,5-Dichloro-N-(2-methyl-4-nitrophenyl)benzenesulfonamide FH535;β-Catenin/Tcf Inhibitor, FH535;FH 535(FH-535);FH535, 98%, a cell-permeable compound that inhibits Wnt/β-catenin and PPAR
• CAS NO: 108409-83-2
• Molecular Formula: C13H10Cl2N2O4S
• Molecular Weight: 361.2005
• Product Categories: Aromatics;Inhibitors;Intermediates & Fine Chemicals;Pharmaceuticals;API
• Mol File: 108409-83-2.mol
• Article Data: 0

Chemical Properties

• Melting Point: 141-142℃
• Boiling Point: 526.3±60.0 °C(Predicted)
• Appearance: /
• Density: 1.566±0.06 g/cm3(Predicted)
• Storage Temp.: 2-8°C
• Solubility: DMSO: soluble20mg/mL
• PKA: 5.83±0.10(Predicted)
• Stability: Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
• CAS DataBase Reference: FH 535(CAS DataBase Reference)
• NIST Chemistry Reference: FH 535(108409-83-2)
• EPA Substance Registry System: FH 535(108409-83-2)

Safety Data

• Hazard Codes: Xi
• Statements: 41
• Safety Statements: 36
• WGK Germany: 3
• Hazardous Substances Data: 108409-83-2(Hazardous Substances Data)

Usage

Description

FH-535 (108409-83-2) suppresses Wnt/β-catenin signaling. It antagonizes PPARγ and PPARδ ligand-dependent activation which is mediated by inhibition of recruitment of the coactivators β-catenin and GRIP1 but not the corepressors NCoR and SMRT.1?Inhibits the migration and growth of breast cancer cell lines2 as well as colon, lung and liver cell lines1. FH-535 is a useful tool for probing the involvement of Wnt signaling pathway.3,4

Chemical Properties

Dark Yellow Solid

Uses

Different sources of media describe the Uses of 108409-83-2 differently. You can refer to the following data:
1. A cell-permeable compound that inhibits Wnt/β-catenin and PPAR
2. Antagonizes Wnt/β-catenin/Tcf-mediated transcription as well as PPARγ and δ activity. Able to inhibit the recruitment of the co-activators β-catenin and GRIP1, both of which are activators for both pathways.

Biochem/physiol Actions

FH535 has the ability to block the development of colon cancer cells. It can change several cancer-associated biological processes. FH535 can also prevent PARylation of Axin2 in osteosarcoma cells.

References

1) Handeli et al. (2008), A small-molecule inhibitor of Tcf/beta-catenin signaling down-regulates PPARgamma and PPARdelta activities; Mol. Cancer Ther., 7 521 2) Iida et al. (2012), FH535 inhibited migration and growth of breast cancer cells; PLoS One, 7 e44418 3) Frewer et al. (2013), A role for WISP2 in colorectal cancer cell invasion and motility; Cancer Genomics Proteomics, 10 187 4) Polk et al. (2012), FH535 potentiation of cigarette smoke condensate cytotoxicity is associated with changes in β-catenin and EGR-1 signaling; Int. J. Toxicol., 31 380 5) Morita and Hayashi (2018), Tumor Progression is Mediated by Thymosin-β4 through a TGFβ/MRTF Signaling Axis; Mol. Cancer Res. 16(5) 880