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2,3,5-Triphenyltetrazolium bromide (TPTZ) is a chemical compound that serves as a redox indicator in biochemical assays, characterized by its ability to change color from colorless to red upon reduction by dehydrogenases in living cells. This property makes TPTZ a valuable tool for assessing cellular viability and the activity of mitochondrial dehydrogenases, which are crucial for cellular metabolism and energy production.

1096-80-6

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1096-80-6 Usage

Uses

Used in Biochemical Assays:
2,3,5-Triphenyltetrazolium bromide is used as a redox indicator for measuring cellular viability and mitochondrial dehydrogenase activity. Its color change from colorless to red upon reduction by dehydrogenases allows for the assessment of cell health and metabolic activity in various biological and medical research applications.
Used in the MTT Assay:
In the MTT assay, 2,3,5-Triphenyltetrazolium bromide is used as a colorimetric substrate to evaluate the cytotoxicity of compounds and to assess cell proliferation. The reduction of TPTZ to its red formazan product by active dehydrogenases in living cells correlates with the number of viable cells, providing a quantitative measure of cell survival and growth.
Used in Microbiology:
2,3,5-Triphenyltetrazolium bromide is used in microbiology as a metabolic indicator to differentiate certain bacterial species based on their metabolic activity. The reduction of TPTZ to its red formazan product by the metabolic activity of bacteria allows for the identification and classification of different bacterial species in microbiological research.

Check Digit Verification of cas no

The CAS Registry Mumber 1096-80-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,0,9 and 6 respectively; the second part has 2 digits, 8 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 1096-80:
(6*1)+(5*0)+(4*9)+(3*6)+(2*8)+(1*0)=76
76 % 10 = 6
So 1096-80-6 is a valid CAS Registry Number.
InChI:InChI=1/C19H16N4.BrH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H,(H,20,21);1H

1096-80-6SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 10, 2017

Revision Date: Aug 10, 2017

1.Identification

1.1 GHS Product identifier

Product name 2,3,5-triphenyl-1H-tetrazol-1-ium,bromide

1.2 Other means of identification

Product number -
Other names 2,3,5-triphenyl-tetrazolium,bromide

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:1096-80-6 SDS

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