111344-55-9Relevant articles and documents
Porphyrinic MOF Film for Multifaceted Electrochemical Sensing
Elsner, Martin,Fischer, Roland A.,Hou, Shujin,Li, Weijin,Mukherjee, Soumya,Zhou, Zhenyu
, p. 20551 - 20557 (2021)
Electrochemical sensors are indispensable in clinical diagnosis, biochemical detection and environmental monitoring, thanks to their ability to detect analytes in real-time with direct electronic readout. However, electrochemical sensors are challenged by sensitivity—the need to detect low concentrations, and selectivity—to detect specific analytes in multicomponent systems. Herein, a porphyrinic metal-organic framework (PP-MOF), Mn-PCN-222 is deposited on a conductive indium tin oxide (ITO) surface. It affords Mn-PCN-222/ITO, a versatile voltammetric sensor able to detect redox-active analytes such as inorganic ions, organic hazardous substances and pollutants, including nitroaromatics, phenolic and quinone-hydroquinone toxins, heavy metal ions, biological species, as well as azo dyes. As a working electrode, the high surface area of Mn-PCN-222/ITO enables high currents, and therefore leverages highly sensitive analysis. The metalloporphyrin centre facilitates analyte-specific redox catalysis to simultaneously detect more than one analyte in binary and ternary systems allowing for detection of a wide array of trace pollutants under real-world conditions, most with high sensitivity.
Catalytic antibodies. Circular dichroism and UV-Vis studies of antibody-metalloporphyrin interactions
Keinan,Benory,Sinha,Sinha-Bagchi,Eren,Eshhar,Green
, p. 5433 - 5438 (2008/10/08)
Circular dichroism and UV-vis measurements were used to study the interaction between several water-soluble metalloporphyrins and monoclonal antibodies (MABs) that were elicited against a structurally related dihydroxytin-(IV) porphyrin, 1a. Some of the MAB-metalloporphyrin complexes studied were previously shown to mimic hemoprotein-like activity, such as catalytic epoxidation of styrene by iodosobenzene. MAB-metalloporphyrin complex formation is usually accompanied by significant bathochromic shift and hypo-/hyperchromicity changes of the absorption maxima in the porphyrin Soret band region. Induced CD spectra in the same region (350-450 nm) are detected upon complex formation. They follow Beer's law, exhibit isosbestic behavior, and show no significant change in the general shape of the Cotton effect as a function of the antibody:porphyrin ratio. Signal intensity (ellipticity) increases with increasing antibody:metalloporphyrin ratio, reaching an upper limit that represents saturation complexation, all indicating a specific 1:1 binding phenomenon. Strong dependence of both signal shape and signal intensity upon the specific metal and antibody were observed, suggesting that induced CD may serve as a powerful tool for the unequivocal differentiation of two seemingly identical MAB clones. In cases of intense induced CD signals, it is possible to obtain MAB-metalloporphyrin dissociation constants using a titration curve where ellipticity is measured as a function of the antibody:porphyrin ratio and presented as a Scatchard plot. Both the UV-vis absorbance and induced CD spectrum of the metalloporphyrin-MAB complex remain unchanged over a broad pH range between 6 and 11, indicating remarkable stability of these complexes and reflecting the dominant role of electrostatic interactions between the hapten carboxylate groups and the antibody combining site.
