1132819-83-0Relevant academic research and scientific papers
Synthesis and identification of hydroxylated metabolites of the anti-estrogenic agent cyclofenil
Gaertner, Peter,Hofbauer, Karin,Reichel, Christian,Geisendorfer, Thomas,Gmeiner, Guenter
experimental part, p. 958 - 964 (2009/05/07)
The detection of metabolites of the anti-estrogenic substance cyclofenil, listed on the World Anti-Doping Agency (WADA) Prohibited List since 2004 is described. Target substances are hydroxylated metabolites, bearing an aliphatic hydroxyl group either in the 2-, 3- or 4-position of the aliphatic ring, in addition to the phenolic functions on the aromatic rings. Structural identification used NMR as well as high-resolution mass spectrometry after nano-electrospray ionisation (ESI). Unambiguous detection of all three synthesised cyclofenil metabolites M1-M3 was done using gas chromatography for separation and electron ionisation mass spectrometry for detection of the per-silylated compounds in comparison with a reference urine deriving from an excretion study within the WADA 2007 Educational Programme. Copyright
