1146952-00-2Relevant academic research and scientific papers
2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor
Pontiggia, Rodrigo,Pontiggia, Osvaldo,Simian, Marina,Montserrat, Javier M.,Engels, Joachim W.,Iribarren, Adolfo M.
supporting information; experimental part, p. 2806 - 2808 (2010/08/04)
A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor α protein (ER-α), and transfection experiments in MCF-7 cell line are also presented.
