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115-40-2

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115-40-2 Usage

Chemical Properties

Crystalline

Uses

Different sources of media describe the Uses of 115-40-2 differently. You can refer to the following data:
1. As indicator, pH 5.2 yellow; pH 6.8 purple.
2. It is used as a pH indicator and to measure serum albumin concentrations. It is used as a pH indicator (5.2-6.8 : yellow to blue-purple) and also used in spectrophotometric determination of serum-albumin.
3. Bromocresol purple has been used as a pH indicator in the yeast culture media. It has been used as a pH indicator in the culture media of Geobacillus stearothermophilus subsp. Calidolactis.

Definition

ChEBI: A member of the class of 2,1-benzoxathioles that is 2,1-benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3-bromo-4-hydroxy-5-methylphenyl groups. A hydrophilic dye that is used as a pH indicator and to measure serum albumin concentrations.

General Description

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Purification Methods

Dissolve the dye in aqueous 5% NaHCO3 solution and precipitate it from a hot solution by dropwise addition of aqueous HCl. Repeat this until the UV/VIS-extinction did not increase at 419nm. It can also be recrystallised from *benzene. It is an indicator: at pH 5.2 (yellow) and pH 6.8 (purple). [Beilstein 19/3 V 460.]

Check Digit Verification of cas no

The CAS Registry Mumber 115-40-2 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 1,1 and 5 respectively; the second part has 2 digits, 4 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 115-40:
(5*1)+(4*1)+(3*5)+(2*4)+(1*0)=32
32 % 10 = 2
So 115-40-2 is a valid CAS Registry Number.
InChI:InChI=1/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

115-40-2 Well-known Company Product Price

  • Brand
  • (Code)Product description
  • CAS number
  • Packaging
  • Price
  • Detail
  • Alfa Aesar

  • (A19530)  Bromocresol Purple   

  • 115-40-2

  • 10g

  • 274.0CNY

  • Detail
  • Alfa Aesar

  • (A19530)  Bromocresol Purple   

  • 115-40-2

  • 50g

  • 627.0CNY

  • Detail
  • Alfa Aesar

  • (A19530)  Bromocresol Purple   

  • 115-40-2

  • 250g

  • 1633.0CNY

  • Detail
  • Fluka

  • (32642)  BromocresolPurple  indicator, Reag. Ph. Eur.

  • 115-40-2

  • 32642-5G

  • 391.95CNY

  • Detail
  • Fluka

  • (32642)  BromocresolPurple  indicator, Reag. Ph. Eur.

  • 115-40-2

  • 32642-25G

  • 1,193.40CNY

  • Detail
  • Fluka

  • (32642)  BromocresolPurple  indicator, Reag. Ph. Eur.

  • 115-40-2

  • 32642-1KG

  • 16,029.00CNY

  • Detail
  • Sigma-Aldrich

  • (114375)  BromocresolPurple  BioReagent, suitable for indicator, Dye content 90 %

  • 115-40-2

  • 114375-5G

  • 499.59CNY

  • Detail
  • Sigma-Aldrich

  • (114375)  BromocresolPurple  BioReagent, suitable for indicator, Dye content 90 %

  • 115-40-2

  • 114375-25G

  • 1,429.74CNY

  • Detail
  • Sigma

  • (17490)  BromocresolPurple  for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)

  • 115-40-2

  • 17490-5G

  • 276.12CNY

  • Detail
  • Sigma

  • (17490)  BromocresolPurple  for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)

  • 115-40-2

  • 17490-10G

  • 465.66CNY

  • Detail
  • Sigma

  • (17490)  BromocresolPurple  for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)

  • 115-40-2

  • 17490-50G

  • 1,467.18CNY

  • Detail

115-40-2SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 10, 2017

Revision Date: Aug 10, 2017

1.Identification

1.1 GHS Product identifier

Product name bromocresol purple

1.2 Other means of identification

Product number -
Other names Bromocresol Purple

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:115-40-2 SDS

115-40-2Synthetic route

2-[Bis-(3-bromo-4-hydroxy-5-methyl-phenyl)-methyl]-benzenesulfonic acid; compound with triethyl-amine

2-[Bis-(3-bromo-4-hydroxy-5-methyl-phenyl)-methyl]-benzenesulfonic acid; compound with triethyl-amine

A

bromcresol purple
115-40-2

bromcresol purple

B

triethylamine
121-44-8

triethylamine

Conditions
ConditionsYield
In acetonitrile at 25℃; Equilibrium constant; other solvents;
furan-3-boronic acid
55552-70-0

furan-3-boronic acid

bromcresol purple
115-40-2

bromcresol purple

C29H22O7S

C29H22O7S

Conditions
ConditionsYield
With bis-triphenylphosphine-palladium(II) chloride; potassium phosphate In water; N,N-dimethyl-formamide at 100℃; Inert atmosphere;65%
4-carboxyphenylboronic acid
14047-29-1

4-carboxyphenylboronic acid

bromcresol purple
115-40-2

bromcresol purple

5'-[3-(3-bromo-4-hydroxy-5-methyl-phenyl)-1,1-dioxo-1,3-dihydro-1λ6-benzo[c][1,2]oxathiol-3-yl]-2'-hydroxy-3'-methyl-biphenyl-4-carboxylic acid

5'-[3-(3-bromo-4-hydroxy-5-methyl-phenyl)-1,1-dioxo-1,3-dihydro-1λ6-benzo[c][1,2]oxathiol-3-yl]-2'-hydroxy-3'-methyl-biphenyl-4-carboxylic acid

Conditions
ConditionsYield
With potassium phosphate; cross-linked resin-captured palladium In water at 120℃; for 0.166667h; Suzuki coupling reaction; microwave irradiation;57%
With potassium phosphate; tetrabutylammomium bromide; palladium diacetate In N,N-dimethyl-formamide at 80℃; for 18h; Suzuki cross-coupling;43%
bromcresol purple
115-40-2

bromcresol purple

4-pyridineboronic acid pinacol ester
181219-01-2

4-pyridineboronic acid pinacol ester

bis(4-pyridine)cresol violet sultone

bis(4-pyridine)cresol violet sultone

Conditions
ConditionsYield
With potassium phosphate; tetrakis(triphenylphosphine) palladium(0) In water; N,N-dimethyl-formamide at 100℃; for 6h; Inert atmosphere;45%
bromcresol purple
115-40-2

bromcresol purple

triethylamine
121-44-8

triethylamine

2-[Bis-(3-bromo-4-hydroxy-5-methyl-phenyl)-methyl]-benzenesulfonic acid; compound with triethyl-amine

2-[Bis-(3-bromo-4-hydroxy-5-methyl-phenyl)-methyl]-benzenesulfonic acid; compound with triethyl-amine

Conditions
ConditionsYield
In acetonitrile at 25℃; Equilibrium constant; other solvents;
bromcresol purple
115-40-2

bromcresol purple

eszopiclone
138729-47-2

eszopiclone

C17H17ClN6O3*C21H16Br2O5S

C17H17ClN6O3*C21H16Br2O5S

Conditions
ConditionsYield
In aq. buffer Acidic conditions;
4-(5H-dibenzo[a,d]cyclohepten-5-ylidene)-1-methylpiperidine hydrochloride

4-(5H-dibenzo[a,d]cyclohepten-5-ylidene)-1-methylpiperidine hydrochloride

bromcresol purple
115-40-2

bromcresol purple

C21H16Br2O5S*C21H21N

C21H16Br2O5S*C21H21N

Conditions
ConditionsYield
In aq. acetate buffer
tri-n-butyl(vinyl)tin
7486-35-3

tri-n-butyl(vinyl)tin

bromcresol purple
115-40-2

bromcresol purple

A

C23H19BrO5S

C23H19BrO5S

B

C25H22O5S

C25H22O5S

Conditions
ConditionsYield
With tetrakis(triphenylphosphine) palladium(0) In N,N-dimethyl-formamide at 80℃;
bromcresol purple
115-40-2

bromcresol purple

carbetapentane citrate
23142-01-0

carbetapentane citrate

C20H31NO3*C21H16Br2O5S

C20H31NO3*C21H16Br2O5S

Conditions
ConditionsYield
In chloroform; cyclohexane

115-40-2Upstream product

115-40-2Downstream Products

115-40-2Related news

Research articleHigh-efficiency extraction of Bromocresol Purple (cas 115-40-2) dye and heavy metals as chromium from industrial effluent by adsorption onto a modified surface of zeolite: Kinetics and equilibrium study08/31/2019

Tannery industrial effluent is rich in heavy metals and basic dyes as bromocresol purple (BCP), poses an economic problem and a serious danger to the environment. This research had evaluated the importance of the adsorption properties of a modified clinoptilolite (CL) (a type of zeolite) for the...detailed

Short communicationIdentification of two Bromocresol Purple (cas 115-40-2) binding sites on human serum albumin08/28/2019

BackgroundIn clinical settings, human serum albumin (HSA) is widely quantitated on the basis of change in the color of bromocresol purple (BCP) bound to HSA. However, the binding sites of BCP on HSA are hitherto unknown. We have identified these sites by performing binding experiments in which B...detailed

Third order nonlinear optical studies of Bromocresol Purple (cas 115-40-2) in liquid and solid media08/27/2019

The quest for materials exhibiting high nonlinear refraction and absorption are required for photonic device application. In this paper we report a simplified Z-scan technique, to study the optical nonlinearity of the dye bromocresol purple in methanol and ethanol for different concentration and...detailed

Immobilization of Bromocresol Purple (cas 115-40-2) in Inorganic-Organic Sol-Gel Thin Film with Presence of Anionic and Non-ionic Surfactants☆08/26/2019

Sol-gel materials known as organically modified silicates (ORMOSILs) offer interesting features such as chemical and mechanical stability. In this paper VTES (vinyltriethoxysilane) and TEOS (tetraalkoxysilane) are mixed in 3:1 ratio. Sol-gel solution was prepared by hydrolysis process of precurs...detailed

Determination of the ground- and excited-state dipole moments of Bromocresol Purple (cas 115-40-2) in protic and aprotic solvents08/25/2019

Although it has been widely recognized that hydrogen bonds play a significant role in the photophysics of molecules, this phenomenon has rarely been applied to the solvatochromic method for determination of dipole moments. The difference in the dipole moment between the ground and excited state ...detailed

Using polyvinyl chloride dyed with Bromocresol Purple (cas 115-40-2) in radiation dosimetry08/24/2019

Polyvinyl chloride (PVC) dyed with bromocresol purple was investigated as a high-dose radiation dosimeter. The absorbance at 417 nm depends linearly on the dose below 50 kGy. The response depends neither on dose rate nor on the irradiation temperature. The effects of post-irradiation storage in ...detailed

Reversible pH-sensitive element based on Bromocresol Purple (cas 115-40-2) immobilized into the polymethacrylate matrix08/22/2019

Reversible pH-sensitive element based on bromocresol purple immobilized into the polymethacrylate matrix was developed. Applications of the pH-sensitive element for continuous monitoring pH of aqueous solution were demonstrated.detailed

115-40-2Relevant articles and documents

A SPECTROPHOTOMETRIC STUDY OF SYSTEMS: SULFOPHTHALEIN DYES-TRIETHYLAMINE IN FOUR NON-AQUEOUS SOLVENTS

Nesterowicz, Marianna,Korewa, Ryszard

, p. 1835 - 1841 (2007/10/02)

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