1152-14-3

Usage

Uses

Used in Research and Diagnostics:
Resorufin Acetate is used as a fluorogenic substrate for hydrolytical enzymes, allowing researchers to study enzyme activity and monitor enzymatic reactions in real-time. The hydrolysis of Resorufin Acetate by these enzymes results in the release of the fluorescent Resorufin, which can be easily detected and quantified.
Used in Analytical Chemistry:
In the field of analytical chemistry, Resorufin Acetate is used as a sensitive and reliable indicator for the detection and quantification of certain enzymes, such as esterases and lipases. The fluorescent signal produced upon hydrolysis enables accurate measurement of enzyme activity and can be applied in various assay formats, including high-throughput screening.
Used in Drug Discovery and Development:
Resorufin Acetate is also utilized in drug discovery and development processes, where it serves as a valuable tool for identifying and optimizing enzyme inhibitors. By monitoring the hydrolysis of Resorufin Acetate and the resulting fluorescent signal, researchers can assess the potency and selectivity of potential drug candidates targeting specific enzymes.
Used in Environmental Monitoring:
In environmental monitoring, Resorufin Acetate can be employed as a substrate to detect and measure the presence of pollutant-degrading enzymes in soil and water samples. The fluorescent signal generated upon enzyme-mediated hydrolysis can provide insights into the enzymatic activity and potential for bioremediation in contaminated environments.
Used in Educational Settings:
Resorufin Acetate is also used in educational settings to demonstrate enzyme kinetics and mechanisms to students. The easy-to-observe fluorescent signal allows for a clear and engaging way to illustrate enzyme activity and the principles of enzyme catalysis.

InChI:InChI=1/C12H7NO3.C2H4O2/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9;1-2(3)4/h1-6,14H;1H3,(H,3,4)

Upstream product

• 108-24-7 acetic anhydride 
• 34994-50-8 7-hydroxy-3H-phenoxazin-3-one sodium salt 
• 91569-74-3 4-(4-hydroxy-anilino)-benzene-1,3-diol; hydrochloride 
• 75-36-5 acetyl chloride 
• 635-78-9 resorufin 
• 4947-02-8 N,N-bis-(2,4-diacetoxy-phenyl)-acetamide 
• 108-46-3 recorcinol 
• 550-82-3 resazurin 

Downstream Products

• 54489-84-8 7-hydroxy-2-(4-nitro-anilino)-phenoxazin-3-one 
• 54489-82-6 7-hydroxy-2-(4-methoxy-anilino)-phenoxazin-3-one 
• 54489-81-5 2-(4-bromo-anilino)-7-hydroxy-phenoxazin-3-one 
• 54489-83-7 7-hydroxy-2-(4-methyl-anilino)-phenoxazin-3-one 
• 54489-80-4 2-anilino-7-hydroxy-phenoxazin-3-one 
• 34994-50-8 7-hydroxy-3H-phenoxazin-3-one sodium salt 
• 54489-76-8 7-acetoxy-2-(4-bromo-anilino)-phenoxazin-3-one 
• 54489-75-7 7-acetoxy-2-anilino-phenoxazin-3-one 
• 54489-79-1 7-acetoxy-2-(4-nitro-anilino)-phenoxazin-3-one 
• 54489-77-9 7-acetoxy-2-(4-methoxy-anilino)-phenoxazin-3-one 
• 54489-78-0 7-acetoxy-2-(4-methyl-anilino)-phenoxazin-3-one 

Relevant academic research and scientific papers

Colorimetric and fluorescence analysis of percarbonate and oxygen bleach by perhydrolysis of resorufin ester

Percarbonate is an important oxidant that is widely used in industrial applications and daily life. In this study, a simple colorimetric and fluorescence method for percarbonate determination using resorufin-based probe 1, carrying a pivaloyl protection group as a signaling switch, was developed. Probe 1 exhibited selective and sensitive signaling behavior for percarbonate via perhydrolysis of the pivaloyl group by hydrogen peroxide that was produced from percarbonate, in the presence of commonly encountered metal ions and anions. Among the esters tested as signaling switches, pivalate was optimal owing to its resistance to spontaneous hydrolysis and high signaling speed under the employed conditions. The detection limit for percarbonate was estimated to be 0.45 μM. The probe was applied to the analysis of percarbonate in domestic bleach using a scanner as a field-oriented signal-capturing device.

Set-up and validation of a high throughput screening method for human monoacylglycerol lipase (MAGL) based on a new red fluorescent probe

Monoacylglycerol lipase (MAGL) is a serine hydrolase that has a key regulatory role in controlling the levels of 2-arachidonoylglycerol (2-AG), the main signaling molecule in the endocannabinoid system. Identification of selective modulators of MAGL enables both to provide new tools for investigating pathophysiological roles of 2-AG, and to discover new lead compounds for drug design. The development of sensitive and reliable methods is crucial to evaluate this modulatory activity. In the current study, we report readily synthesized long-wavelength putative fluorogenic substrates with different acylic side chains to find a new probe for MAGL activity. 7-Hydroxyresorufinyl octanoate proved to be the best substrate thanks to the highest rate of hydrolysis and the best Km and Vmax values. In addition, in silico evaluation of substrates interaction with the active site of MAGL confirms octanoate resorufine derivative as the molecule of choice. The well-known MAGL inhibitors URB602 and methyl arachidonylfluorophosphonate (MAFP) were used for the assay validation. The assay was highly reproducible with an overall average Z0 value of 0.86. The fast, sensitive and accurate method described in this study is suitable for low-cost high-throughput screening (HTS) of MAGL modulators and is a powerful new tool for studying MAGL activity.