115853-74-2 Usage
Description
6'-Dimethyl-4'-(N-succinimidyloxycarbonyl)phenyl-10-methyl-acridinium-9-carboxylate methosulfate (DMAE-NHS) is a heterobifunctional crosslinker with chemiluminescent properties. It is designed to connect azide-containing compounds or biomolecules to those with a primary amine, forming a covalent adduct. The molecule features a hydrophilic PEG spacer, which enhances its water solubility and allows for easy modification of amine-containing biomolecules such as antibodies, proteins, and peptides with a terminal alkyne. Additionally, the PEG spacer helps reduce aggregation of modified proteins in solution by adding hydrophilic character.
Uses
Used in Binding Assays:
DMAE-NHS is used as a chemiluminescent agent in binding assays, either alone or when incorporated into liposomes. Its chemiluminescent properties make it a valuable tool for detecting and quantifying specific interactions between biomolecules.
Used in Chemical Biology and Drug Development:
As a heterobifunctional crosslinker, DMAE-NHS is used for the covalent attachment of azide-containing compounds or biomolecules to those with a primary amine. This application is particularly useful in chemical biology and drug development, where it can be employed to create novel conjugates, probe protein-protein interactions, or develop targeted drug delivery systems.
Used in Diagnostics and Imaging:
The chemiluminescent properties of DMAE-NHS make it suitable for use in diagnostic assays and imaging techniques. It can be incorporated into various detection systems to provide a signal upon binding, allowing for the identification and localization of specific biomolecules or molecular interactions.
Used in Research and Development:
DMAE-NHS is a valuable reagent for researchers working with biomolecules, as it enables the creation of novel conjugates and the study of protein-protein interactions. Its versatility in connecting different types of molecules makes it a useful tool for exploring new avenues in biological research and the development of innovative therapeutic strategies.
Used in the Pharmaceutical Industry:
In the pharmaceutical industry, DMAE-NHS can be utilized to develop new drug candidates or improve the delivery and efficacy of existing drugs. Its ability to form covalent adducts with primary amines and azides allows for the creation of targeted drug conjugates, potentially enhancing the specificity and effectiveness of therapeutic agents.
Used in the Biotechnology Industry:
Biotechnological applications can benefit from the use of DMAE-NHS, as it enables the modification of amine-containing biomolecules such as antibodies, proteins, and peptides. This can lead to the development of novel biotechnological products, including engineered enzymes, biosensors, and other bioactive molecules with tailored properties.
Check Digit Verification of cas no
The CAS Registry Mumber 115853-74-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,5,8,5 and 3 respectively; the second part has 2 digits, 7 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 115853-74:
(8*1)+(7*1)+(6*5)+(5*8)+(4*5)+(3*3)+(2*7)+(1*4)=132
132 % 10 = 2
So 115853-74-2 is a valid CAS Registry Number.
115853-74-2Relevant articles and documents
Synthesis of acridinium ester chemiluminescent substrate DMAE.NHS
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, (2017/08/29)
The invention aims to provide a synthesis method of a chemiluminescent substrate namely acridinium ester 9-((4-(((2,5-dioxo-1-pyrrolidinyl)oxy)carbonyl)-2,6-dimethylphenoxyl)carbonyl)-10-methyl acridine onium methyl sulfate (DAME.NHS). The chemical structure of DMAE.NHS is represented in the description. According to the synthesis method, 3,5-dimethyl-4-hydroxyl benzoic acid, N-hydroxyl succinimide, and acridine-9-carbonyl chloride are taken as the raw materials, and after two steps of esterification reactions, the reaction product is subjected to methylation to obtain the target compound. Compared with other synthesis method, the provided synthesis method has the advantages that the steps are few, the method does not need any pricy reagent or solvent, the reaction conditions are mild, the product yield and purity are high, and the synthesis method is suitable for massive production.
