1160931-29-2Relevant academic research and scientific papers
Illuminating a Dark Kinase: Structure-Guided Design, Synthesis, and Evaluation of a Potent Nek1 Inhibitor and Its Effects on the Embryonic Zebrafish Pronephros
Baumann, Georg,Meckel, Tobias,B?hm, Kevin,Shih, Yung-Hsin,Dickhaut, Mirco,Reichardt, Torben,Pilakowski, Johannes,Pehl, Ulrich,Schmidt, Boris
, p. 1265 - 1282 (2021/04/12)
NIMA-related kinase 1 (Nek1) has lately garnered attention for its widespread function in ciliogenesis, apoptosis, and the DNA-damage response. Despite its involvement in various diseases and its potential as a cancer drug target, no directed medicinal chemistry efforts toward inhibitors against this dark kinase are published. Here, we report the structure-guided design of a potent small-molecule Nek1 inhibitor, starting from a scaffold identified by kinase cross-screening analysis. Seven lead compounds were identified in silico and evaluated for their inhibitory activity. The top compound, 10f, was further profiled for efficacy, toxicity, and bioavailability in a zebrafish polycystic kidney disease model. Administration of 10f caused the expansion of fluorescence-labeled proximal convoluted tubules, supporting our hypothesis that Nek1-inhibition causes cystic kidneys in zebrafish embryos. Compound 10f displayed insignificant inhibition in 48 of 50 kinases in a selectivity test panel. The findings provide a powerful tool to further elucidate the function and pharmacology of this neglected kinase.
4-Phenyl-7-azaindoles as potent and selective IKK2 inhibitors
Liddle, John,Bamborough, Paul,Barker, Michael D.,Campos, Sebastien,Cousins, Rick P.C.,Cutler, Geoffrey J.,Hobbs, Heather,Holmes, Duncan S.,Ioannou, Chris,Mellor, Geoff W.,Morse, Mary A.,Payne, Jeremy J.,Pritchard, John M.,Smith, Kathryn J.,Tape, Daniel T.,Whitworth, Caroline,Williamson, Richard A.
scheme or table, p. 2504 - 2508 (2009/12/25)
The synthesis and SAR of a novel series of IKK2 inhibitors are described. Modification around the hinge binding region of the 7-azaindole led to a series of potent and selective inhibitors with good cellular activity.
