1166966-77-3Relevant academic research and scientific papers
Protease probes built from DNA: Multispectral fluorescent DNA-peptide conjugates as caspase chemosensors
Dai, Nan,Guo, Jia,Teo, Yin Nah,Kool, Eric T.
, p. 5105 - 5109 (2011)
Shaken, not stirred: A novel design for protease sensors is described, in which peptides are conjugated to a DNA backbone carrying multiple fluorophores. The multispectral oligodeoxyfluoroside (ODF) fluorophores are strongly quenched by a dabcyl group at the end of each peptide. In vitro and cellular selectivity assays showed that a mixture of three sensors could be used to identify different caspase activities by the fluorescence outcome (see picture). Copyright
Quenched ligand-directed tosylate reagents for one-step construction of turn-on fluorescent biosensors
Tsukiji, Shinya,Wang, Hangxiang,Miyagawa, Masayoshi,Tamura, Tomonori,Takaoka, Yousuke,Hamachi, Itaru
supporting information; experimental part, p. 9046 - 9054 (2009/12/04)
Semisynthetic fluorescent biosensors consisting of a protein framework and a synthetic fluorophore are powerful analytical tools for specific detection of biologically relevant molecules. We report herein a novel method that allows for the construction of
