1193501-24-4Relevant academic research and scientific papers
Azido push-pull fluorogens photoactivate to produce bright fluorescent labels
Lord, Samuel J.,Lee, Hsiao-Lu D.,Samuel, Reichel,Weber, Ryan,Liu, Na,Conley, Nicholas R.,Thompson, Michael A.,Twieg, Robert J.,Moerner
, p. 14157 - 14167 (2011/04/24)
Dark azido push-pull chromophores have the ability to be photoactivated to produce bright fluorescent labels suitable for single-molecule imaging. Upon illumination, the aryl azide functionality in the fluorogens participates in a photochemical conversion to an aryl amine, thus restoring charge-transfer absorption and fluorescence. Previously, we reported that one compound, DCDHF-V-P-azide, was photoactivatable. Here, we demonstrate that the azide-to-amine photoactivation process is generally applicable to a variety of push-pull chromophores, and we characterize the photophysical parameters including photoconversion quantum yield, photostability, and turn-on ratio. Azido push-pull fluorogens provide a new class of photoactivatable singlemolecule probes for fluorescent labeling and super-resolution microscopy. Lastly, we demonstrate that photoactivated push-pull dyes can insert into bonds of nearby biomolecules, simultaneously forming a covalent bond and becoming fluorescent (fluorogenic photoaffinity labeling).
