120534-19-2Relevant academic research and scientific papers
Optimized synthesis of novel prenyl ferulate performed by feruloyl esterases from Myceliophthora thermophila in microemulsions
Antonopoulou, Io,Leonov, Laura,Jütten, Peter,Cerullo, Gabriella,Faraco, Vincenza,Papadopoulou, Adamantia,Kletsas, Dimitris,Ralli, Marianna,Rova, Ulrika,Christakopoulos, Paul
, p. 3213 - 3226 (2017)
Five feruloyl esterases (FAEs; EC 3.1.1.73), FaeA1, FaeA2, FaeB1, and FaeB2 from Myceliophthora thermophila C1 and MtFae1a from M. thermophila ATCC 42464, were tested for their ability to catalyze the transesterification of vinyl ferulate (VFA) with prenol in detergentless microemulsions. Reaction conditions were optimized investigating parameters such as the medium composition, the substrate concentration, the enzyme load, the pH, the temperature, and agitation. FaeB2 offered the highest transesterification yield (71.5?±?0.2%) after 24?h of incubation at 30?°C using 60?mM VFA, 1?M prenol, and 0.02?mg FAE/mL in a mixture comprising of 53.4:43.4:3.2?v/v/v n-hexane:t-butanol:100?mM MOPS-NaOH, pH 6.0. At these conditions, the competitive side hydrolysis of VFA was 4.7-fold minimized. The ability of prenyl ferulate (PFA) and its corresponding ferulic acid (FA) to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals was significant and similar (IC50 423.39?μM for PFA, 329.9?μM for FA). PFA was not cytotoxic at 0.8–100?μM (IC50 220.23?μM) and reduced intracellular reactive oxygen species (ROS) in human skin fibroblasts at concentrations ranging between 4 and 20?μM as determined with the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
