1217308-91-2Relevant academic research and scientific papers
Perchlorotrityl radical-fluorophore conjugates as dual fluorescence and EPR probes for superoxide radical anion
Wang, Jinhua,Dang, Vinh,Zhao, Wei,Lu, Dongning,Rivera, Brian K.,Villamena, Frederick A.,Wang, Peng George,Kuppusamy, Periannan
experimental part, p. 922 - 929 (2010/04/29)
Perchlorotrityl radicals, mono-substituted with a fluorophore using an amide linker of varying chain length, were synthesized and characterized. Electron paramagnetic resonance (EPR) spectroscopic study indicated free-electron coupling with the aromatic hydrogen nuclei and long-range coupling with the methylene hydrogens of the linker group. Reactivity of the fluorophore-conjugated trityls with superoxide radical anion showed quenching of EPR signal and enhancement of fluorescence emission spectrum. This work presents the first example of a perchlorotrityl-fluorophore conjugate that can potentially be employed as a dual probe for the detection of superoxide under oxidative stress-mediated conditions in biological systems.
