1220702-45-3

Upstream product

• 1220702-43-1 1-{5-O-[bis(4-methoxyphenyl)(phenyl)methyl]-2-deoxy-β-D-ribofuranos-1-yl}-5-(4-nitro-1H-imidazol-1-yl)-pyrimidine-2,4-(1H,3H)-dione 
• 124482-92-4 2-cyanoethyl-N,N-(diisopropylamino)chlorophosphine 

Relevant academic research and scientific papers

Synthesis and biophysical properties of oligodeoxynucleotides containing 2′-deoxy-5-(4-nitro-1H-imidazol-1-yl)-β-D-uridine and 2′-deoxy-5-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)-β-D-uridine monomers

Detection of single-nucleotide polymorphisms (SNPs) of biologically relevant DNA and RNA samples remain a scientific and practical challenge. We have synthesized phosphoramidite building blocks and oligodeoxynucleotide probes containing novel 2′-deoxyuridine monomers modified by 5-(4-nitro-1H-imidazol-1-yl; (monomer X) or 2′-deoxy-5-(1,3-dioxo-1H- benzo[de]isoquinolin-2(3H)-yl; monomer Y) substituents. The effects of monomers X and Y on duplex thermal stability, and their capability towards discrimination of single-base mismatches were furthermore studied. Encouraging results were obtained with respect to thermal mismatch discrimination using oligodeoxynucleotides containing monomer X and fluorescence-based discrimination using oligodeoxynucleotides containing monomer Y.