1220982-32-0Relevant academic research and scientific papers
Antioxidant and antiviral activities of silybin fatty acid conjugates
Ga?ák, Radek,Purchartová, Kate?ina,Marhol, Petr,?ivná, Lucie,Sedmera, Petr,Valentová, Kate?ina,Kato, Nobuo,Matsumura, Hiroyo,Kaihatsu, Kunihiro,K?en, Vladimír
experimental part, p. 1059 - 1067 (2010/04/24)
Two selective acylation methods for silybin esterification with long-chain fatty acids were developed, yielding a series of silybin 7-O- and 23-O-acyl-derivatives of varying acyl chain lengths. These compounds were tested for their antioxidant (inhibition of lipid peroxidation and DPPH-scavenging) and anti-influenza virus activities. The acyl chain length is an important prerequisite for both biological activities, as they improved with increasing length of the acyl moiety.
Enzymatic kinetic resolution of silybin diastereoisomers
Monti, Daniela,Gazak, Radek,Marhol, Petr,Biedermann, David,Purchartova, Katerina,Fedrigo, Mirko,Riva, Sergio,Kren, Vladimir
experimental part, p. 613 - 619 (2010/07/13)
In nature, the flavonolignan silybin (1) occurs as a mixture of two diastereomers, silybin A and silybin B, which in a number of biological assays exhibit different activities. A library of hydrolases (lipases, esterases, and proteases) was tested for separating the silybin A and B diastereomers by selective transesterification or by stereoselective alcoholysis of 23-O-acetylsilybin (2). Novozym 435 proved to be the most suitable enzyme for the preparative production of both optically pure silybins A and B by enzymatic discrimination. Gram amounts of the optically pure substances can be produced within one week, and the new method is robust and readily scalable to tens of grams.
