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12284-43-4

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12284-43-4 Usage

Uses

Azurin has been used:in the cytotoxicity and cell viability studies in human osteosarcoma cell linefor the functionalization of silicon nitride cantilevers for interaction studiesfor coating gold surface and insulating functionalized oxide surfaces of silicon oxide and mica

Biochem/physiol Actions

Azurin is a metalloprotein in the family of cupredoxins. It preferentially enters cancer cells over normal cells and induces apoptosis. Azurin has structural similarities to ephrinB2, and in fact binds the ephrin receptor tyrosine kinase EphB2 to initiate cell signaling that is involved in cancer progression. Azurin inhibits autophosphorlyation of the EphB2 tyrosine residue, interfering with upstream cell signaling and contributing to cancer cell growth inhibition.

Purification Methods

Azurin with A625/280 = 0.56 is purified by gel chromatography on G-25 Sephadex with 5mM phosphate pH 7 buffer as eluent [Cho et al. J Phys Chem 91 3690 1987]. It is a blue Cu protein used in biological electron transport, and its reduced form is obtained by adding a slight excess of Na2S2O4. [See Structure and Bonding Springer Verlag, Berlin 23 1 1975.]

Check Digit Verification of cas no

The CAS Registry Mumber 12284-43-4 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,2,2,8 and 4 respectively; the second part has 2 digits, 4 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 12284-43:
(7*1)+(6*2)+(5*2)+(4*8)+(3*4)+(2*4)+(1*3)=84
84 % 10 = 4
So 12284-43-4 is a valid CAS Registry Number.

12284-43-4Upstream product

12284-43-4Downstream Products

12284-43-4Related news

Direct wiring of the AZURIN (cas 12284-43-4) redox center to gold electrodes investigated by protein film voltammetry08/18/2019

We present two surface-immobilization schemes that connect an azurin variant with mutations in the redox cavity to two oligophenylvinyl (OPV) molecular wires, along with an electrochemical study of their electron transfer (ET) properties. OPV embedded within alkanethiol films tether selectively ...detailed

Multifunctional 4-bit biomemory chip consisting of recombinant AZURIN (cas 12284-43-4) variants08/16/2019

We developed a multi-functional 4-bit biomemory chip that consisted of recombinant azurin variants. The azurin was modified to introduce cysteine-residues. In addition, the Cu ion in this recombinant azurin protein was substituted with various other metal ions such as Co, Mn, Fe and Ni ion to al...detailed

Mercury metallation of the copper protein AZURIN (cas 12284-43-4) and structural insight into possible heavy metal reactivity08/14/2019

Mercury(II) metallation of Pseudomonas aeruginosa azurin has been characterized structurally and biochemically. The X-ray crystal structure at 1.5 Å of mercury(II) metallated azurin confirms the coordination of mercury at the copper binding active site and a second surface site. These findings a...detailed

Isomorphic deactivation of a Pseudomonas aeruginosa oxidoreductase: The crystal structure of Ag(I) metallated AZURIN (cas 12284-43-4) at 1.7 Å08/13/2019

Multiple biophysical methods demonstrate that silver effectively metallates Pseudomonas aeruginosa apo-azurin in solution. X-ray crystallography of the silver-modified protein reveals that silver binds to azurin at the traditional copper mediated active site with nearly identical geometry. Cycli...detailed

The interaction of AZURIN (cas 12284-43-4) and C-terminal domain of p53 is mediated by nucleic acids08/12/2019

Azurin is bacterial protein, which was been reported to promote cancer cell death in vitro. The interaction of azurin and p53 is important for the cytotoxic effect of azurin towards cancer cells. In this study, it was found that nucleic acids mediated the interaction of azurin and the C-terminal...detailed

Surface Immobilized His-tagged AZURIN (cas 12284-43-4) as a Model Interface for the Investigation of Vectorial Electron Transfer in Biological Systems08/11/2019

A model system for the electrochemical investigation of vectorial electron transfer in biological systems was designed, assembled and characterized. Gold electrodes, functionalized with a -OCH3 terminated, aromatic self-assembled monolayer, were used as a substrate for the adsorption of variants...detailed

Probing redox reaction of AZURIN (cas 12284-43-4) protein immobilized on hydroxyl-terminated self-assembled monolayers with different lengths08/10/2019

Immobilization of proteins with controlled electron transfer properties is an important task for future biomedical applications. In this work, immobilization of azurin on gold electrodes modified with 6-mercaptohexanol (MCH), and 11-mercaptoundecanol (MUD) self-assembled monolayers (SAMs) was st...detailed

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