Mercury metallation of the copper protein AZURIN (cas 12284-43-4) and structural insight into possible heavy metal reactivity
-
Add time:08/14/2019 Source:sciencedirect.com
Mercury(II) metallation of Pseudomonas aeruginosa AZURIN (cas 12284-43-4) has been characterized structurally and biochemically. The X-ray crystal structure at 1.5 Å of mercury(II) metallated azurin confirms the coordination of mercury at the copper binding active site and a second surface site. These findings are further validated by NMR, Matrix-assisted laser desorption/ionization spectrometry (MALDI), and UV–visible spectroscopic methods indicating copper displacement from the wild-type protein. Bioinformatic analysis has identified homologous human protein domains computationally, and compared them to the structure of azurin, providing a model for human mercury interactions. Study of the mercury–azurin adduct, in combination with other known examples of protein–heavy metal interactions, could provide further insight into the chemical mechanisms of toxicological interactions, leading toward a global understanding of the biological speciation of toxic heavy metals.
We also recommend Trading Suppliers and Manufacturers of AZURIN (cas 12284-43-4). Pls Click Website Link as below: cas 12284-43-4 suppliers
Prev:Isomorphic deactivation of a Pseudomonas aeruginosa oxidoreductase: The crystal structure of Ag(I) metallated AZURIN (cas 12284-43-4) at 1.7 Å
Next:Recombinant AZURIN (cas 12284-43-4)-CdSe/ZnS hybrid structures for nanoscale resistive random access memory device) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Direct wiring of the AZURIN (cas 12284-43-4) redox center to gold electrodes investigated by protein film voltammetry08/18/2019
- High-throughput molecular profiling of a P-cadherin overexpressing breast cancer model reveals new targets for the anti-cancer bacterial protein AZURIN (cas 12284-43-4)08/17/2019
- Multifunctional 4-bit biomemory chip consisting of recombinant AZURIN (cas 12284-43-4) variants08/16/2019
- Recombinant AZURIN (cas 12284-43-4)-CdSe/ZnS hybrid structures for nanoscale resistive random access memory device08/15/2019
- Isomorphic deactivation of a Pseudomonas aeruginosa oxidoreductase: The crystal structure of Ag(I) metallated AZURIN (cas 12284-43-4) at 1.7 Å08/13/2019
- The interaction of AZURIN (cas 12284-43-4) and C-terminal domain of p53 is mediated by nucleic acids08/12/2019
- Surface Immobilized His-tagged AZURIN (cas 12284-43-4) as a Model Interface for the Investigation of Vectorial Electron Transfer in Biological Systems08/11/2019
- Probing redox reaction of AZURIN (cas 12284-43-4) protein immobilized on hydroxyl-terminated self-assembled monolayers with different lengths08/10/2019
- Electrochemical atomic force microscopy reveals potential stimulated height changes of redox responsive Cu-AZURIN (cas 12284-43-4) on gold08/09/2019