1245724-75-7Relevant academic research and scientific papers
Biomimetic Iron Complex Achieves TET Enzyme Reactivity**
Carell, Thomas,Daumann, Lena J.,Jonasson, Niko S. W.,Korytiaková, Eva,Schmidl, David
supporting information, p. 21457 - 21463 (2021/08/23)
The epigenetic marker 5-methyl-2′-deoxycytidine (5mdC) is the most prevalent modification to DNA. It is removed inter alia via an active demethylation pathway: oxidation by Ten-Eleven Translocation 5-methyl cytosine dioxygenase (TET) and subsequent removal via base excision repair or direct demodification. Recently, we have shown that the synthetic iron(IV)-oxo complex [FeIV(O)(Py5Me2H)]2+ (1) can serve as a biomimetic model for TET by oxidizing the nucleobase 5-methyl cytosine (5mC) to its natural metabolites. In this work, we demonstrate that nucleosides and even short oligonucleotide strands can also serve as substrates, using a range of HPLC and MS techniques. We found that the 5-position of 5mC is oxidized preferably by 1, with side reactions occurring only at the strand ends of the used oligonucleotides. A detailed study of the reactivity of 1 towards nucleosides confirms our results; that oxidation of the anomeric center (1′) is the most common side reaction.
Quantification of the sixth DNA base hydroxymethylcytosine in the brain
Muenzel, Martin,Globisch, Daniel,Brueckl, Tobias,Wagner, Mirko,Welzmiller, Veronika,Michalakis, Stylianos,Mueller, Markus,Biel, Martin,Carell, Thomas
supporting information; experimental part, p. 5375 - 5377 (2010/10/18)
Mind over matter: LC-MS has allowed the amount of the post-replicatively formed DNA base 5hydroxymethylcytosine (see picture; left) to be quantified in brain tissue. The nucleoside is most abundant in areas that are associated with higher cognitive functions, and its content in mouse hippocampi seems to increase with age. The new method enables hydroxymethylcytosine to be quantified with unprecedented accuracy. (Figure Presented)
