1246943-82-7

Upstream product

• 6066-82-6 1-hydroxy-pyrrolidine-2,5-dione 
• 146368-11-8 C₃₃H₄₀N₂O₈S₂ 
• 146368-11-8 1-(5-carboxypentyl)-2-[3,3-dimethyl-5-sulfo-1-(4-sulfobutyl)-2,3-dihydro-1H-indol-2-yliden]-1,3-pentadienyl-3,3-dimethyl-3H-indolium-5-sulfonate 
• 105832-38-0 O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate 
• 146368-11-8 C₃₃H₄₀N₂O₈S₂ 
• 917979-28-3 3H-indolium-2-[(1E,3E,5E)-5-[1-(5-carboxypentyl)-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadienyl]-1-ethyl-3,3-dimethyl-5-sulfo-, inner salt 

Downstream Products

• 1396463-63-0 BMV₀₁₁ 
• 1169606-46-5 MRS₅₂₁₈ 
• 1609570-41-3 C₁₀₂H₁₃₁N₁₁O₁₇S₃ 
• 1427311-83-8 C₅₈H₇₄N₇O₂₃P₃S₃ 
• 1427311-82-7 C₅₈H₇₄N₇O₂₄P₃S₂ 

Relevant academic research and scientific papers

POLYMERIC SYSTEMS AND USES THEREOF IN THERANOSTIC APPLICATIONS

Polymeric systems useful for theranostic applications are disclosed. The polymeric systems comprise a fluorescent or fluorogenic moiety and a therapeutically active agent, each attached to the same or different polymeric moiety. The polymeric systems are designed such that a fluorescent signal is generated in response to a chemical event, preferably upon contacting an analyte (e.g., an enzyme) that is over- expressed in a diseased tissue or organ. Probes useful for inclusion in such polymeric systems, processes of preparing such probes and the polymeric systems, and uses thereof in diagnostic and/or theranostic applications are also disclosed.

Bioconjugatable azo-based dark-quencher dyes: Synthesis and application to protease-activatable far-red fluorescent probes

We describe the efficient synthesis and one-step derivatization of novel, nonfluorescent azo dyes based on the Black Hole Quencher-3 (BHQ-3) scaffold. These dyes were equipped with various reactive and/or bioconjugatable groups (azido, α-iodoacetyl, keton

Water soluble indodicarbocyanine dyes based on 2,3-dimethyl-3-(4- sulfobutyl)-3H-indole-5-sulfonic acid

A series of mono-reactive, water-soluble, indodicarbocyanine dyes based on 2,3-dimethyl-3-(4-sulfobutyl)-3H-indole-5-sulfonic acid was synthesized. These dyes contain two aromatic sulfo groups and in addition to (up to 3) sulfoalkyl groups in the indolenine end groups. The impact of the number and position of sulfobutyl groups on the spectral properties and photostabilities of the dyes free in solutions and after binding to an antibody (IgG) was investigated. These dyes have absorption maxima between 649 and 653 nm, molar absorptivities between 240,000 and 256,000 M-1 cm-1, emission maxima from 668 to 672 nm, and fluorescence quantum yields between 28 and 32% in free form and up to 43% when bound to IgG. Independent of the number of sulfo groups, these dyes exhibit no aggregation in water at concentrations up to 5 × 10-5 M. The number of sulfoalkyl groups was found to directly correlate with the fluorescence quantum yields and photostabilities of the IgG conjugated dyes.

Intracellular pH measurements using perfluorocarbon nanoemulsions

We report the synthesis and formulation of unique perfluorocarbon (PFC) nanoemulsions enabling intracellular pH measurements in living cells via fluorescent microscopy and flow cytometry. These nanoemulsions are formulated to readily enter cells upon coincubation and contain two cyanine-based fluorescent reporters covalently bound to the PFC molecules, specifically Cy3-PFC and CypHer5-PFC conjugates. The spectral and pH-sensing properties of the nanoemulsions were characterized in vitro and showed the unaltered spectral behavior of dyes after formulation. In rat 9L glioma cells loaded with nanoemulsion, the local pH of nanoemulsions was longitudinally quantified using optical microscopy and flow cytometry and displayed a steady decrease in pH to a level of 5.5 over 3 h, indicating rapid uptake of nanoemulsion to acidic compartments. Overall, these reagents enable real-time optical detection of intracellular pH in living cells in response to pharmacological manipulations. Moreover, recent approaches for in vivo cell tracking using magnetic resonance imaging (MRI) employ intracellular PFC nanoemulsion probes to track cells using 19F MRI. However, the intracellular fate of these imaging probes is poorly understood. The pH-sensing nanoemulsions allow the study of the fate of the PFC tracer inside the labeled cell, which is important for understanding the PFC cell loading dynamics, nanoemulsion stability and cell viability over time.

A simple FRET-based modular design for diagnostic probes

In recent years, there has been a massive effort to develop molecular probes with optical modes of action. Probes generally produce detectable signals based on changes in fluorescence properties. Here, we demonstrate the potential of self-immolative molecular adaptors as a platform for Turn-On probes based on the FRET technique. The probe is equipped with identical fluorophore pairs or a fluorophore/quencher FRET pair and a triggering substrate. Upon reaction of the analyte of interest with the triggering substrate, the self-immolative adaptor spontaneously releases the two dye molecules to break off the FRET effect. As a result, a new measurable fluorescent signal is generated. The fluorescence obtained can be used to quantify the analyte. The modular structure of the probe design will allow the preparation of various chemical probes based on the FRET activation technique.

A universal and ready-to-use heterotrifunctional cross-linking reagent for facile synthetic access to sophisticated bioconjugates

We describe for the first time, the synthesis and some bioconjugation applications of an original heterotrifunctional cross-linking reagent (also named tripod) bearing three different bioorthogonal functional groups which are fully compatible amongst themselves. Contrary to the first generation tripod recently reported by us (Org. Biomol. Chem., 2008, 6, 3065), the use of an azido group instead of the nucleophile-sensitive active carbamate moiety enables us to reach the targeted chemical orthogonality without the use of temporary aminooxy- and thiol protecting groups. Thus, the preparation of sophisticated bioconjugates through the sequential derivatisation of the tripod by means of copper-mediated 1,3-dipolar cycloaddition, oxime ligation and aqueous compatible mild thiol-alkylation reactions, is significantly simpler and more convenient. The chemoselective bioconjugation protocols were optimised through the preparation of FRET cassettes based on cyanine and/or xanthene fluorescent dye pairs and subsequent anchoring to fragile biomolecules. The applicability of this universal cross-linking reagent was also illustrated by the preparation of biochips suitable for aflatoxin B1 detection through the SPIT-FRI method.

Aminopropargyl derivative of terpyridine-bis(methyl-enamine) tetraacetic acid chelate of europium (Eu (TMT)-AP3): A new reagent for fluorescent labelling of proteins and peptides

The synthesis and photophysical properties of a new terpyridine-based europium(iii) chelate (Eu (TMT)-AP3) designed for peptide and protein labelling in aqueous solution phase is described. In order to obtain a stable, easy to handle, versatile

Aryldithioethyloxycarbonyl (Ardec): A new family of amine protecting groups removable under Mild reducing conditions and their applications to peptide synthesis

The development of phenyl-dithioethyloxycarbonyl (Phdec) and 2-pyridyldithioethyloxycarbonyl (Pydec) protecting groups, which are thiollabile urethanes, is described. These new disulfide-based protecting groups were introduced onto the ε-amino group of L-

SITE-SPECIFIC LABELLING OF PROTEINS USING CYANINE DYE REPORTERS

Disclosed are compounds of formula (I):in which D is a dye selected from a cyanine dye or a derivative thereof; B is an affinity tag;F comprises a target bonding group selected from a carboxylic acid thioester group and a 1,2-aminothiol group; M is a g