1259016-41-5Relevant academic research and scientific papers
Acceptor specificity in the transglycosylation reaction using Endo-M
Tomabechi, Yusuke,Odate, Yuki,Izumi, Ryuko,Haneda, Katsuji,Inazu, Toshiyuki
experimental part, p. 2458 - 2463 (2011/01/04)
To determine the structural specificity of the glycosyl acceptor of the transglycosylation reaction using endo-β-N-acetylglucosaminidase (ENGase) (EC 3.2.1.96) from Mucor hiemalis (Endo-M), several acceptor derivatives were designed and synthesized. The narrow regions of the 1,3-diol structure from the 4- to 6-hydroxy functions of GlcNAc were found to be essential for the transglycosylation reaction using Endo-M. Furthermore, it was determined that Endo-M strictly recognizes a 1,3-diol structure consisting of primary and secondary hydroxyl groups.
