127556-39-2Relevant academic research and scientific papers
SYNTHESIS AND USE OF AN AMINOQUINOLINONE DERIVATIVE FOR THE FLUOROMETRIC DETERMINATION OF OXYTOCINASE
Maskova, Hana P.,Kokotos, George,Tzougraki, Chryssa,Barth, Tomislav
, p. 2802 - 2808 (2007/10/02)
A new fluorogenic substrate for the determination of the activity of human serum oxytocinase-cystine aminopeptidase (EC 3.4.11.3), H-Cys(Bzl)-NH-Meq, has been synthesized.The affinity of H-Cys(Bzl)-NH-Meq to oxytocinase was by two orders higher than that of the usuallyemployed chromogenic substrates.The Michaelis constant of oxytocinase for this substrate was within the range of the optimium pH (7.0 - 7.5) 2.3E-6 moll-1, i.e. in the region of the affinity of the natural substrate, oxytocin.The concentration of dimethylsulfoxide used for the enzyme solubilizationof H-Cys(Bzl)-NH-Meq (0.4percent) did not influence adversely the course of the enzyme reaction as in the case of chromogenic substrates, where the concentration of the organic solvent exceeded 3percent
