129203-60-7

Basic information

• Product Name: IBERIOTOXIN
• Synonyms: IBTX;IBTX (SCORPION, BUTHUS TAMULUS);IBERIOTOXIN;IBERIOTOXIN, BUTHUS TAMULUS;IBERIOTOXIN SCORPION BUTHUS TAMULUS;PGLU-PHE-THR-ASP-VAL-ASP-CYS-SER-VAL-SER-LYS-GLU-CYS-TRP-SER-VAL-CYS-LYS-ASP-LEU-PHE-GLY-VAL-ASP-ARG-GLY-LYS-CYS-MET-GLY-LYS-LYS-CYS-ARG-CYS-TYR-GLN [DISULFIDE BRIDGES: 7-28, 13-3, 17-3];RLBTX;PYR-FTDVDCSVSKECWSVCKDLFGVDRGKCMGKKCRCYQ
• CAS NO: 129203-60-7
• Molecular Formula: C179H274N50O55S7
• Molecular Weight: 4230.84786
• Product Categories: Ion Channel Modulating Peptides;Potassium Channel ModulatorsPeptides for Cell Biology;Monovalent Ion Channels;Potassium Channel ModulatorsCell Signaling and Neuroscience;Scorpion;Toxins and Venoms;Voltage-gated Ion Channels;Enzyme Inhibitors;Enzyme Inhibitors by Type;Other;Potassium channel
• Mol File: 129203-60-7.mol
• Article Data: 0

Chemical Properties

• Boiling Point: °Cat760mmHg
• Flash Point: °C
• Appearance: /
• Density: g/cm³
• Storage Temp.: −20°C
• Solubility: H2O: 1 mg/mL, clear, colorless
• CAS DataBase Reference: IBERIOTOXIN(CAS DataBase Reference)
• NIST Chemistry Reference: IBERIOTOXIN(129203-60-7)
• EPA Substance Registry System: IBERIOTOXIN(129203-60-7)

Safety Data

• Hazard Codes: T+
• Statements: 26/27/28
• Safety Statements: 28-36/37-45
• RIDADR: UN 3462 6.1/PG 2
• WGK Germany: 3
• RTECS: NH6107500
• F: 3-10
• Hazardous Substances Data: 129203-60-7(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 129203-60-7 differently. You can refer to the following data:
1. Selective probe for high-conductance calcium-activated potassium channels.
2. Iberiotoxin is a selective and reversible inhibitor of high-conductance calcium-activated potassium channel. A toxin purified from the Eastern Indian red scorpion Buthus tamulus.
3. Iberiotoxin, recombinant from Mesobuthus tamulus has been used as an inhibitor of large-conductance calcium-activated K+ channels to study its effect on the NLRP3 (nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3) inflammasome activation in bone marrow-derived macrophages (BMDMs). It has also been used to block Ca2+- activated K+ channels to determine interleukin(IL)-1β in BMDCs following inflammasome activation.

Biological Activity

iberiotoxin is a 37 amino acid peptidyl toxin isolated from the scorpion mesobuthus tamulus and was shown to selectively block large conductance ca2+-activated k+ channels(bkca) in smooth muscle cells.in single channel recordings, iberiotoxin can reversibly block outward currents mediated by bkca in excised membrane patches from bovine aortic smooth muscle with an ic50 value of 250 pm. it is also a noncompetitive, partial inhibitor of charybdotoxin binding in bovine aortic sarcolemmal membrane vesicles (ki = 250 pm).[1]. galvez a, gimenez-gallego g, reuben j p, et al. purification and characterization of a unique, potent, peptidyl probe for the high conductance calcium-activated potassium channel from venom of the scorpion buthus tamulus. the journal of biological chemisty, 1990, 265(19): 11083-11090.

Biochem/physiol Actions

The single chain peptide iberiotoxin (IbTX) is a selective and reversible inhibitor of high-conductance calcium-activated potassium channels (PK,Ca). It occurs naturally in the venom of the scorpion Buthus tamulus. IbTX also modulates the binding of charybdotoxin (CbTX) to smooth muscle sarcolemmal membranes in a non-competitive manner. IbTX is similar in size to CbTX and shares considerable sequence homology with CbTX. However, IbTX differs in its overall charge in having one fewer basic and four more acidic residues than CbTX.

InChI:InChI=1/C179H274N50O55S7/c1-87(2)65-111-155(261)208-112(66-93-33-15-13-16-34-93)146(252)195-77-133(239)225-139(88(3)4)172(278)213-117(71-136(244)245)158(264)199-100(44-31-62-190-178(186)187)144(250)193-75-131(237)198-102(40-22-27-58-181)148(254)219-124(167(273)205-109(56-64-285-12)145(251)194-76-132(238)197-101(39-21-26-57-180)147(253)200-105(43-25-30-61-184)151(257)220-123-81-286-288-83-125(221-152(258)106(203-166(123)272)45-32-63-191-179(188)189)169(275)210-113(68-95-46-48-97(234)49-47-95)156(262)206-110(177(283)284)50-53-129(185)235)82-287-291-86-128(168(274)202-104(42-24-29-60-183)150(256)212-116(70-135(242)243)159(265)207-111)224-175(281)142(91(9)10)228-164(270)121(79-231)216-157(263)115(69-96-74-192-99-38-20-19-37-98(96)99)211-170(276)126-84-289-290-85-127(171(277)217-122(80-232)165(271)227-141(90(7)8)174(280)218-120(78-230)163(269)201-103(41-23-28-59-182)149(255)204-108(154(260)222-126)52-55-134(240)241)223-160(266)118(72-137(246)247)214-173(279)140(89(5)6)226-162(268)119(73-138(248)249)215-176(282)143