13014-90-9 Usage
Uses
Used in Biochemistry and Cell Biology Research:
2,3,5-Triphenyltetrazolium iodide is used as a redox indicator for assessing cell viability and metabolic activity. It is reduced by actively metabolizing cells to form a red formazan product, which can be detected and quantified using spectrophotometry or microscopy. The reduction of TTI by cells is dependent on the presence of NADH and NADPH, two important coenzymes involved in cellular respiration and metabolism.
Used in Viability Assays:
In viability assays, 2,3,5-Triphenyltetrazolium iodide is used as a metabolic activity indicator to determine the number of living cells in a sample. The formazan product formed upon reduction by metabolically active cells serves as a measure of cell viability, allowing researchers to evaluate the effectiveness of treatments or the impact of experimental conditions on cell survival.
Used in Drug Screening and Toxicity Testing:
2,3,5-Triphenyltetrazolium iodide is employed in drug screening and toxicity testing to assess the impact of potential therapeutic agents on cell viability. By measuring the reduction of TTI to formazan, researchers can evaluate the cytotoxic effects of drugs and identify compounds with potential therapeutic benefits.
Used in Tissue Engineering and Regenerative Medicine:
In tissue engineering and regenerative medicine, 2,3,5-Triphenyltetrazolium iodide is utilized to evaluate the metabolic activity of engineered tissues and cell cultures. The formazan product formed upon reduction by cells can provide insights into the viability and functionality of the engineered tissues, aiding in the optimization of culture conditions and scaffold design.
Used in Environmental Toxicity Assessment:
2,3,5-Triphenyltetrazolium iodide is used in environmental toxicity assessment to evaluate the impact of pollutants and contaminants on aquatic and terrestrial organisms. By measuring the reduction of TTI to formazan in exposed organisms, researchers can assess the toxicity of environmental samples and monitor the health of ecosystems.
Check Digit Verification of cas no
The CAS Registry Mumber 13014-90-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,3,0,1 and 4 respectively; the second part has 2 digits, 9 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 13014-90:
(7*1)+(6*3)+(5*0)+(4*1)+(3*4)+(2*9)+(1*0)=59
59 % 10 = 9
So 13014-90-9 is a valid CAS Registry Number.
InChI:InChI=1/C19H15N4.HI/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1
